Anti-Von Willebrand Factor 抗体 (ab9378)
製品の概要
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製品名
Anti-Von Willebrand Factor antibody
Von Willebrand Factor 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Von Willebrand Factor -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Fr, IHC-P, Flow Cyt, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse
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免疫原
Factor VIII related antigen isolated from human plasma.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Liquid antiserum -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab9378 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | 1/100. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | 1/50 - 1/100. | |
| Flow Cyt | 1/250. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 309 kDa). |
ターゲット情報
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機能
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. -
組織特異性
Plasma. -
関連疾患
Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. -
配列類似性
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
ドメイン
The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. -
翻訳後修飾
All cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated. -
細胞内局在
Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules. - Information by UniProt
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参照データベース
- Entrez Gene: 7450 Human
- Entrez Gene: 22371 Mouse
- Omim: 613160 Human
- SwissProt: P04275 Human
- SwissProt: Q8CIZ8 Mouse
- Unigene: 440848 Human
- Unigene: 22339 Mouse
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別名
- Coagulation factor VIII antibody
- Coagulation factor VIII VWF antibody
- F8VWF antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (ab9378)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Von Willebrand Factor with ab9378.
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Western blot - Anti-Von Willebrand Factor antibody (ab9378)Anti-Von Willebrand Factor antibody (ab9378) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 309 kDa
Observed band size: 250 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa, 50 kDa, 65 kDa. We are unsure as to the identity of these extra bands.
Many bands of varying sizes can be seen on Western blots, perhaps indicating differential processing by ADAMTS13 and other enzymes -
Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody (ab9378)This image is courtesy of an Abreview submitted by Dr. Mahesh ShivananjappaCentral Panel: ab9378 staining Von Willebrand Factor in human PMN cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% TritonX-100 + 2% BSA in PBS and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37°C. An Alexa Fluor® 568-conjugated Goat polyclonal to rabbit IgG, dilution 1/100, was used as secondary antibody.
Top panel: Nuclei counterstained with DAPI (blue).
Bottom panel: Overlay -
Flow Cytometry - Anti-Von Willebrand Factor antibody (ab9378)Image courtesy of Dr Mahesh Shivananjappa by Abreview.ab9378 staining Von Willebrand Factor in Human platelet cells by Flow cytometry.
P : Permeabilized;
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.
US : Unstained, Red Peak; IGG RB : IgG Rabbit (Isotype Control), Blue Peak;
VWF : Von Willebrand Factor antibody, Green peak. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (ab9378)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining Von Willebrand Factor with ab9378.
プロトコール
参考文献
ab9378 は 14 報の論文で使用されています。
- Geng W et al. Exosomes from miRNA-126-modified ADSCs promotes functional recovery after stroke in rats by improving neurogenesis and suppressing microglia activation. Am J Transl Res 11:780-792 (2019). PubMed: 30899379
- Vattulainen-Collanus S et al. Bone morphogenetic protein signaling is required for RAD51-mediated maintenance of genome integrity in vascular endothelial cells. Commun Biol 1:149 (2018). PubMed: 30272025
- Ling L et al. bFGF promotes Sca-1+ cardiac stem cell migration through activation of the PI3K/Akt pathway. Mol Med Rep 17:2349-2356 (2018). PubMed: 29207135
- Smeda M et al. Nitric oxide deficiency and endothelial-mesenchymal transition of pulmonary endothelium in the progression of 4T1 metastatic breast cancer in mice. Breast Cancer Res 20:86 (2018). PubMed: 30075800
- Smeda M et al. Dual antiplatelet therapy with clopidogrel and aspirin increases mortality in 4T1 metastatic breast cancer-bearing mice by inducing vascular mimicry in primary tumour. Oncotarget 9:17810-17824 (2018). PubMed: 29707148
