Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (optic nerve)
Permeabilization
Yes - 0.25% tritonX100
Specification
optic nerve
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: invitrogen
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Works in human samples, as advertised.
We tested in pig with the same protocol. We have not gotten positive staining above our negative controls so far.
Abcam response
Abcam has not tested ab6994 with pig samples and does not know how it should have performed. This researcher has received a credit for testing ab6994 with pig samples via our Abtrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 30 2021