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Immunohistochemistry (Frozen sections) abreview for Anti-Von Willebrand Factor antibody

Excellent
Abreviews
Go to datasheet (ab11713)
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Mouse Tissue sections (skin dermis)
Specification
skin dermis
Permeabilization
No
Fixative
Acetone

Other product details

Incubation time
45 minute(s) · Temperature: 24°C · Diluent: 1% BSA in PBS
Dilution
1/100

Secondary antibody

Name
Abcam antibody:Donkey Anti-Sheep IgG H&L (Alexa Fluor­ 488)
Dilution
1/1000

Additional data

Additional Notes
Higher concentration may get stronger staining. vWF and CD31 are 100% overlap, great antibody for IF.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 May 30 2014