Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.2% saponin
Sample
Mouse Cell (skin)
Specification
skin
Preparation
Cell harvesting/tissue preparation method: Wilson L, Fathke C, Isik F. Tissue dispersion and flow cytometry for the cellular analysis of wound healing. BioTechniques 2002: 32: 548-551.
Sample buffer: 2%BSA/PBS
Sample buffer: 2%BSA/PBS
Other product details
Incubation time
30 minute(s)
Dilution
1/200
Additional data
Additional Notes
1/200, 1/400, 1/800, 1/1000, and 1/2000 dilutions tested, all negative results
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 14 2014