Anti-Vitronectin/S-Protein 抗体 (ab272865)
Key features and details
- Rabbit polyclonal to Vitronectin/S-Protein
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Vitronectin/S-Protein antibody
Vitronectin/S-Protein 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Vitronectin/S-Protein -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment within Human Vitronectin/S-Protein. The exact sequence is proprietary. Center region.
Database link: P04004 -
ポジティブ・コントロール
- WB: Mouse brain tissue extract. HepG2 conditioned medium. IHC-P: Human colon and lung carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272865の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/500 - 1/3000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. -
組織特異性
Plasma. -
配列類似性
Contains 4 hemopexin repeats.
Contains 1 SMB (somatomedin-B) domain. -
ドメイン
The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin. -
翻訳後修飾
Sulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium. -
細胞内局在
Secreted, extracellular space. - Information by UniProt
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参照データベース
- Entrez Gene: 7448 Human
- Entrez Gene: 22370 Mouse
- Omim: 193190 Human
- SwissProt: P04004 Human
- SwissProt: P29788 Mouse
- Unigene: 2257 Human
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別名
- Complement S Protein antibody
- Epibolin antibody
- S Protein antibody
see all
画像
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Anti-Vitronectin/S-Protein antibody (ab272865) at 1/1000 dilution + Mouse brain tissue extracts at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Paraffin-embedded human colon carcinoma tissue stained for Vitronectin/S protein using ab272865 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Paraffin-embedded human lung carcinoma tissue stained for Vitronectin/S protein using ab272865 at 1/1000 dilution in immunohistochemical analysis. Antigen retrieval using Citrate buffer, pH 6.0, 15 min.
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All lanes : Anti-Vitronectin/S-Protein antibody (ab272865) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HepG2 conditioned medium
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272865 は論文での使用が確認できていません。