Anti-Vitronectin/S-Protein 抗体 (ab272865)
Key features and details
- Rabbit polyclonal to Vitronectin/S-Protein
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Vitronectin/S-Protein antibody
Vitronectin/S-Protein 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Vitronectin/S-Protein -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseHuman
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免疫原
Recombinant fragment within Human Vitronectin/S-Protein. The exact sequence is proprietary. Center region.
Database link: P04004 -
ポジティブ･コントロール
- WB: Mouse brain tissue extract. HepG2 conditioned medium. IHC-P: Human colon carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272865 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Mouse
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. -
組織特異性
Plasma. -
配列類似性
Contains 4 hemopexin repeats.
Contains 1 SMB (somatomedin-B) domain. -
ドメイン
The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin. -
翻訳後修飾
Sulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium. -
細胞内局在
Secreted, extracellular space. - Information by UniProt
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参照データベース
- Entrez Gene: 7448 Human
- Entrez Gene: 22370 Mouse
- Omim: 193190 Human
- SwissProt: P04004 Human
- SwissProt: P29788 Mouse
- Unigene: 2257 Human
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別名
- Complement S Protein antibody
- Epibolin antibody
- S Protein antibody
see all
画像
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Western blot - Anti-Vitronectin/S-Protein antibody (ab272865)Anti-Vitronectin/S-Protein antibody (ab272865) at 1/1000 dilution + Mouse brain tissue extracts at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody (ab272865)Paraffin-embedded human colon carcinoma tissue stained for Vitronectin/S protein using ab272865 at 1/500 dilution in immunohistochemical analysis. Antigen retrieval using EDTA based, pH 8.0 buffer, 15 minutes.
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Western blot - Anti-Vitronectin/S-Protein antibody (ab272865)All lanes : Anti-Vitronectin/S-Protein antibody (ab272865) at 1/1000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HepG2 conditioned medium
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272865 は論文での使用が確認できていません。