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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Vitamin D Receptor 抗体 [EPR4552] - ChIP Grade (ab109234)

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Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
  • ChIP - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
  • Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
  • Immunoprecipitation - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
  • Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
  • Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
  • Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
  • Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4552] to Vitamin D Receptor - ChIP Grade
  • Suitable for: WB, IP, ChIP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade
    Vitamin D Receptor 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4552] to Vitamin D Receptor - ChIP Grade

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IP, ChIPmore details
    適用なし: Flow Cyt,ICC/IF or IHC-P

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa, U-937, T-47D and SKBR-3 cell lysates; Rat and mouse kidney tissue lysates. ChIP: Chromatin prepared from T-47D cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109234の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

For unpurified use at 1/1000 - 1/10000.
IP
1/30.
ChIP
Use 5 µg for 25 µg of chromatin.
特記事項
WB
1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

For unpurified use at 1/1000 - 1/10000.
IP
1/30.
ChIP
Use 5 µg for 25 µg of chromatin.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.

  • 関連疾患

    Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.

  • 配列類似性

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • ドメイン

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 1 25 dihydroxyvitamin D3 receptor antibody
    • 1 antibody
    • 1,25 dihydroxyvitamin D3 receptor antibody
    • 1,25-@dihydroxyvitamin D3 receptor antibody
    • 25-dihydroxyvitamin D3 receptor antibody
    • Member 1 antibody
    • NR1I1 antibody
    • Nuclear receptor subfamily 1 group I member 1 antibody
    • PPP1R163 antibody
    • Protein phosphatase 1, regulatory subunit 163 antibody
    • VDR antibody
    • VDR_HUMAN antibody
    • Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody
    • Vitamin D hormone receptor antibody
    • Vitamin D nuclear receptor variant 1 antibody
    • Vitamin D receptor antibody
    • Vitamin D3 receptor antibody
    see all

画像

  • Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
    Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
    All lanes : Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/1000 dilution

    Lane 1 : Wild-type HeLa lysate
    Lane 2 : Vitamin D Receptor knockout HeLa lysate
    Lane 3 : U-937 lysate
    Lane 4 : SH-SY5Y lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 48 kDa



    Lanes 1-4: Merged signal (red and green). Green - ab109234 observed at 50 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab109234 Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade was shown to specifically react with Vitamin D Receptor in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265430 (knockout cell lysate ab257796) was used. Wild-type and Vitamin D Receptor knockout samples were subjected to SDS-PAGE. ab109234 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • ChIP - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
    ChIP - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)

    Chromatin was prepared from T-47D cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25 µg of chromatin, 5 µg of ab109234 (red), and 20 µL of protein A/G sepharose beads slurry (10 µL of sepharose A beads + 10 µL of sepharose G beads). 5 μg of rabbit normal IgG was added to the beads as a control sample (grey). The immunoprecipitated DNA was quantified by real time PCR (SYBR Green chemistry) with primers to c-Fos.

  • Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
    Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
    Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/5000 dilution (purified) + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
    Immunoprecipitation - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)

    Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate, 10 μg
    Lane 2(+): T-47D whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109234 in T-47D whole cell lysate

    Ab109234 immunoprecipitating Vitamin D receptor in T-47D whole cell lysates. Capture antibody was used at a 1/30 dilution (2 μg in 0.35 mg lysates). For western blotting, primary antibody was used as ab109234 at 1/1000 dilution (0.62 μg/mL). VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

  • Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
    Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
    Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/5000 dilution (purified) + Mouse kidney tissue lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
    Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
    Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/1000 dilution (purified) + Rat kidney tissue lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
    Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
    All lanes : Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/1000 dilution (unpurified)

    Lane 1 : T47D cell lysate
    Lane 2 : SKBR-3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 48 kDa
    Observed band size: 48 kDa

  • Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
    Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)

参考文献 (23)

ab109234 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109234 は 23 報の論文で使用されています。

  • Yang Y  et al. Vitamin D protects glomerular mesangial cells from high glucose-induced injury by repressing JAK/STAT signaling. Int Urol Nephrol 53:1247-1254 (2021). PubMed: 33942213
  • Cai Q  et al. Inflammation-Associated Senescence Promotes Helicobacter pylori-Induced Atrophic Gastritis. Cell Mol Gastroenterol Hepatol 11:857-880 (2021). PubMed: 33161156
  • Bass JJ  et al. The mechanisms of skeletal muscle atrophy in response to transient knockdown of the vitamin D receptor in vivo. J Physiol 599:963-979 (2021). PubMed: 33258480
  • Paukovcekova S  et al. Enhanced Antiproliferative Effect of Combined Treatment with Calcitriol and All-Trans Retinoic Acid in Relation to Vitamin D Receptor and Retinoic Acid Receptor a Expression in Osteosarcoma Cell Lines. Int J Mol Sci 21:N/A (2020). PubMed: 32916897
  • Hu Z  et al. VDR activation attenuate cisplatin induced AKI by inhibiting ferroptosis. Cell Death Dis 11:73 (2020). PubMed: 31996668
View all Publications for this product

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