遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Vitamin D Receptor 抗体 [EPR4552] - ChIP Grade (ab109234)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4552] to Vitamin D Receptor - ChIP Grade
  • Suitable for: WB, IP, ChIP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade
    Vitamin D Receptor 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4552] to Vitamin D Receptor - ChIP Grade

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IP, ChIPmore details
    適用なし: Flow Cyt,ICC/IF or IHC-P

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human Vitamin D Receptor aa 1-100. The exact sequence is proprietary.
    Database link: P11473
    (Peptide available as ab198626)

  • ポジティブ･コントロール

    • WB: HeLa, U-937, T-47D and SKBR-3 cell lysates; Rat and mouse kidney tissue lysates. ChIP: Chromatin prepared from T-47D cells.

  • 特記事項

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).

For unpurified use at 1/1000 - 1/10000.
IP 1/30.
ChIP Use 5 µg for 25 µg of chromatin.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • ターゲット情報

    • 機能

      Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.

    • 関連疾患

      Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.

    • 配列類似性

      Belongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.

    • ドメイン

      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

    • 細胞内局在

      Nucleus.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • 1 25 dihydroxyvitamin D3 receptor antibody
      • 1 antibody
      • 1,25 dihydroxyvitamin D3 receptor antibody
      • 1,25-@dihydroxyvitamin D3 receptor antibody
      • 25-dihydroxyvitamin D3 receptor antibody
      • Member 1 antibody
      • NR1I1 antibody
      • Nuclear receptor subfamily 1 group I member 1 antibody
      • PPP1R163 antibody
      • Protein phosphatase 1, regulatory subunit 163 antibody
      • VDR antibody
      • VDR_HUMAN antibody
      • Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody
      • Vitamin D hormone receptor antibody
      • Vitamin D nuclear receptor variant 1 antibody
      • Vitamin D receptor antibody
      • Vitamin D3 receptor antibody
      see all

    画像

    • Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
      Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
      All lanes : Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/1000 dilution

      Lane 1 : Wild-type HeLa lysate
      Lane 2 : Vitamin D Receptor knockout HeLa lysate
      Lane 3 : U-937 lysate
      Lane 4 : SH-SY5Y lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 48 kDa



      Lanes 1-4: Merged signal (red and green). Green - ab109234 observed at 50 kDa. Red - loading control ab8245 observed at 37 kDa.

       ab109234 Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade was shown to specifically react with Vitamin D Receptor in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265430 (knockout cell lysate ab257796) was used. Wild-type and Vitamin D Receptor knockout samples were subjected to SDS-PAGE. ab109234 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

       

    • ChIP - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
      ChIP - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)

      Chromatin was prepared from T-47D cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25 µg of chromatin, 5 µg of ab109234 (red), and 20 µL of protein A/G sepharose beads slurry (10 µL of sepharose A beads + 10 µL of sepharose G beads). 5 μg of rabbit normal IgG was added to the beads as a control sample (grey). The immunoprecipitated DNA was quantified by real time PCR (SYBR Green chemistry) with primers to c-Fos.

    • Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
      Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
      Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/5000 dilution (purified) + HeLa cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
      Immunoprecipitation - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)

      Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate, 10 μg
      Lane 2(+): T-47D whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109234 in T-47D whole cell lysate

      Ab109234 immunoprecipitating Vitamin D receptor in T-47D whole cell lysates. Capture antibody was used at a 1/30 dilution (2 μg in 0.35 mg lysates). For western blotting, primary antibody was used as ab109234 at 1/1000 dilution (0.62 μg/mL). VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

    • Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
      Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
      Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/5000 dilution (purified) + Mouse kidney tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
      Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
      Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/1000 dilution (purified) + Rat kidney tissue lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
      Western blot - Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
      All lanes : Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234) at 1/1000 dilution (unpurified)

      Lane 1 : T47D cell lysate
      Lane 2 : SKBR-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa

    • Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)
      Anti-Vitamin D Receptor antibody [EPR4552] - ChIP Grade (ab109234)

    参考文献 (17)

    ab109234 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab109234 は 17 報の論文で使用されています。

    • Hu Z  et al. VDR activation attenuate cisplatin induced AKI by inhibiting ferroptosis. Cell Death Dis 11:73 (2020). PubMed: 31996668
    • Salhab A  et al. 25(OH) D3 alleviate liver NK cytotoxicity in acute but not in chronic fibrosis model of BALB/c mice due to modulations in vitamin D receptor. BMC Gastroenterol 20:102 (2020). PubMed: 32276660
    • Cui P  et al. Vitamin D Attenuates Hypoxia-Induced Injury in Rat Primary Neuron Cells through Downregulation of the Dual Oxidase 1 (DUOX1) Gene. Med Sci Monit 26:e925350 (2020). PubMed: 32712621
    • Zhao G  et al. Adaptive homeostasis of the vitamin D-vitamin D nuclear receptor axis in 8-methoxypsoralen-induced hepatotoxicity. Toxicol Appl Pharmacol 362:150-158 (2019). PubMed: 30419252
    • Zhang J  et al. p62 functions as an oncogene in colorectal cancer through inhibiting apoptosis and promoting cell proliferation by interacting with the vitamin D receptor. Cell Prolif N/A:e12585 (2019). PubMed: 30793399
    View all Publications for this product

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