Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH/3T3, untransfected, mVDR-transfected)
Specification
NIH/3T3, untransfected, mVDR-transfected
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
1% BSA, 5% HS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 20°C · Diluent: 1% BSA, 5% HS in 1x PBS
Secondary antibody
Name
Non-Abcam antibody was used: anti rb IgG, Jackson ImmunoResearch
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Additional Notes
cells were transfected with expression vector for murine VDR
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Miss. A Uhmann
Verified customer
投稿 Dec 03 2009