Application
Western blot
Sample
Mouse Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing (4–15% Mini-PROTEAN® TGX™ Precast Protein Gels)
Loading amount
30 µg
Specification
Lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA TBST
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 second(s)
Bands
Specific: 55 kDa Non-specific: 50, 75 kDa
Positive control
Lung Homogenate
Additional data
Additional Notes
Lane 1: Lung Homogenate Sample 1
Lane 2: Lung Homogenate Sample 2
Lane 3: Precision Plus Protein™ Unstained Protein Standards (3 ul)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Daniel Tan
Verified customer
投稿 Oct 28 2020