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  1. Link

    vitamin-d-receptor-antibody-ab3508.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger
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Anti-Vitamin D Receptor 抗体 (ab3508)

  • Datasheet
  • SDS
Reviews (5)Q&A (3)References (38)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Receptor antibody (ab3508)

    Key features and details

    • Rabbit polyclonal to Vitamin D Receptor
    • Suitable for: IHC-P
    • Reacts with: Mouse, Rat, Chicken, Human
    • Isotype: IgG

    こちらの製品もご検討ください

    二次抗体
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    タンパク質
    Product image
    Recombinant Human Vitamin D Receptor protein (ab82068)

    関連製品

    製品の概要

    • 製品名

      Anti-Vitamin D Receptor antibody
      Vitamin D Receptor 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to Vitamin D Receptor
    • 由来種

      Rabbit
    • アプリケーション

      適用あり: IHC-Pmore details
    • 種交差性

      交差種: Mouse, Rat, Chicken, Human
      交差が予測される動物種: Cow, Pig, Zebrafish, Saguinus oedipus
    • 免疫原

      Synthetic peptide corresponding to Human Vitamin D Receptor aa 395-413.
      Sequence:

      EEHSKQYRCLSFQPECSMK


      Database link: P11473
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      Preservative: 0.05% Sodium azide
    • Concentration information loading...
    • 精製度

      Whole antiserum
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Zinc Finger
      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • Nuclear Hormone Receptors
      • Vitamin D Receptor
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • Nuclear Receptors
      • Vitamin D Receptor
      • Cancer
      • Signal transduction
      • Nuclear signaling
      • Nuclear hormone receptors
      • Other

    関連製品

    • ChIP Related Products

      • Rabbit Anti-Mouse IgG H&L (ab46540)
      • ChIP Kit (ab500)
    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Vitamin D Receptor protein (ab82068)

    アプリケーション

    Our Abpromise guarantee covers the use of ab3508 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    IHC-P 1/2000 - 1/4000.

    ターゲット情報

    • 機能

      Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
    • 関連疾患

      Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.
    • 配列類似性

      Belongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • ドメイン

      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • 細胞内局在

      Nucleus.
    • Target information above from: UniProt accession P11473 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 7421 Human
      • Entrez Gene: 22337 Mouse
      • Entrez Gene: 24873 Rat
      • Omim: 601769 Human
      • SwissProt: P11473 Human
      • SwissProt: P48281 Mouse
      • SwissProt: P13053 Rat
      • Unigene: 524368 Human
      • Unigene: 245084 Mouse
      • Unigene: 10911 Rat
      see all
    • 別名

      • 1 25 dihydroxyvitamin D3 receptor antibody
      • 1 antibody
      • 1,25 dihydroxyvitamin D3 receptor antibody
      • 1,25-@dihydroxyvitamin D3 receptor antibody
      • 25-dihydroxyvitamin D3 receptor antibody
      • Member 1 antibody
      • NR1I1 antibody
      • Nuclear receptor subfamily 1 group I member 1 antibody
      • PPP1R163 antibody
      • Protein phosphatase 1, regulatory subunit 163 antibody
      • VDR antibody
      • VDR_HUMAN antibody
      • Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody
      • Vitamin D hormone receptor antibody
      • Vitamin D nuclear receptor variant 1 antibody
      • Vitamin D receptor antibody
      • Vitamin D3 receptor antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Receptor antibody (ab3508)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Receptor antibody (ab3508)

      Ab3508 staining Human normal jejunum. Staining is localized to the nucleus.
      Left panel: with primary antibody at 1/2000. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (38)

    ab3508 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3508 は 38 報の論文で使用されています。

    • Qin T  et al. Prediction of the mechanisms of action of Shenkang in chronic kidney disease: A network pharmacology study and experimental validation. J Ethnopharmacol 246:112128 (2020). PubMed: 31386888
    • Piotrowska A  et al. Antiproliferative activity of side-chain truncated vitamin D analogs (PRI-1203 and PRI-1204) against human malignant melanoma cell lines. Eur J Pharmacol 881:173170 (2020). PubMed: 32445704
    • Yang R  et al. 1,25-Dihydroxyvitamin D protects against age-related osteoporosis by a novel VDR-Ezh2-p16 signal axis. Aging Cell 19:e13095 (2020). PubMed: 31880094
    • Jaratsittisin J  et al. Activity of vitamin D receptor agonists against dengue virus. Sci Rep 10:10835 (2020). PubMed: 32616772
    • Lee JS  et al. Claudin-1 expression decreases with increasing pathological grade in actinic keratosis and may be a marker of high-risk actinic keratosis. Clin Exp Dermatol 44:483-490 (2019). PubMed: 30315595
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-8 of 8 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Vitamin D Receptor antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (NIH/3T3, untransfected, mVDR-transfected)
    Specification
    NIH/3T3, untransfected, mVDR-transfected
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton X-100
    Blocking step
    1% BSA, 5% HS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
    Read More

    Miss. A Uhmann

    Verified customer

    投稿 Dec 03 2009

    Western blot abreview for Anti-Vitamin D Receptor antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (Hodgkin lymphoma cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    Hodgkin lymphoma cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 02 2020

    Western blot abreview for Anti-Vitamin D Receptor antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Lung)
    Gel Running Conditions
    Reduced Denaturing (4–15% Mini-PROTEAN® TGX™ Precast Protein Gels)
    Loading amount
    30 µg
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Daniel Tan

    Verified customer

    投稿 Oct 28 2020

    Question

    I recently ordered Anti Vitamin D receptor antibody – ChIP grade ab3508, on the data sheet it says concentration not determined. The lot number is GR41724-6. Do you happen to know the concentration, and do you have any recommendations for a protocol? I have used the antibody at a 1/2000 1/4000 dilution (if the ab is 1mg/ml) and I get really high background staining with low nuclear staining. I use citrate buffer pH 6.0 for HIER for 40 minutes in a vegetable steamer then 30 min room temperature cool down. I use peroxidase block 10 min with 3%h2o2 and 15 min protein block using vector animal free blocker. Then I incubate the primary antibody for 30 minutes, do another protein block for 15 minutes and use MACH 4 biocare polymer for 30 minutes. Also I have tried to incubate the primary overnight and use a rabbit alkaline phosphatase secondary antibody with Vector red chromogen and I get no staining. Thus is there anything you can recommend to assist my VDR stain efforts? Thanks for your time and have a great day.

    Read More

    Abcam community

    Verified customer

    Asked on Dec 13 2012

    Answer

    Thank you for contacting us.

    Since this is a whole antiserum antibody, the concentration has not been determined. Typically the total protein concentration for these antibodies can range from 1 to 10 mg/ml (you could split the difference and estimate 5 mg/ml).

    You may want to remove the additional protein blocking step after the primary antibody incubation. Plus he antigen retrieval step may be a bit too long. Typically 10 mins boiling in a microwave followed by 20 min cooling works well. Also, since this is a nuclear antigen, you should permeabilize with Triton X-100 if you're not already. What species are your samples from and what is the tissue type (i.e. rat spleen)?

    I'm listing some references below which have cited the use of this antibody in IHC:

    Arch Biochem Biophys. 2010 Feb 15;494(2):166-77.
    "Identification of a highly specific and versatile vitamin D receptor antibody."
    Author(s): Wang Y, Becklund BR, DeLuca HF
    Number of Citations: 1

    Biogerontology. 2008 Apr;9(2):109-18.
    "Vitamin D receptor levels and binding are reduced in aged rat intestinal subcellular fractions."
    Author(s): González Pardo V, Boland R, de Boland AR
    Number of Citations: 1

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Abcam Scientific Support

    Answered on Dec 13 2012

    Question

    Inquiry: Hi, I was wondering if a concentration is available for this antibody. If not, do you have any specific recommendations as to how much should be used in for a ChIP experiment? Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 17 2012

    Answer

    Thank you very much for contacting us with your enquiry.

    Ab3508 is an unpurified whole serum antibody, and as there are other proteins in the serum the concentration of specific IgG can not be measured. This antibody has been used in ChIP in 4 known publications, though it's not tested in ChIP in our own lab. I've looked through the references (attached) and in "Ab3508 ChIP 1" and the protocol references of "Ab3508 ChIP 2, the authors used 5 uL of ab3508 per chromatin pellet.

    I am sorry that we don't have more information, but I hope this will be useful. If you have any further questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

    Read More

    Abcam Scientific Support

    Answered on Oct 17 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Vitamin D Receptor antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Whole embryo)
    Specification
    Whole embryo
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 14 2009

    Western blot abreview for Anti-Vitamin D Receptor antibody - ChIP Grade

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - nuclear (NIH/3T3, untransfected, VDR-transfected)
    Loading amount
    10 µg
    Specification
    NIH/3T3, untransfected, VDR-transfected
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    I-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C
    Read More

    Miss. A Uhmann

    Verified customer

    投稿 Dec 01 2009

    Question

    What dilution was used with this antibody in IP?

    Read More

    Abcam community

    Verified customer

    Asked on May 16 2006

    Answer

    Thank you for your enquiry. The resources I have available indicate that 1 ul of ab3508 should be a sufficient amount for immunoprecipitation assays. I would recommend using a minimum of 100 ug of sample. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on May 18 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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