Anti-VISTA 抗体 [BLR035F] (ab243891)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR035F] to VISTA
- Suitable for: WB, ICC, IHC-P, IP, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-VISTA antibody [BLR035F]
VISTA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [BLR035F] to VISTA -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC, IHC-P, IP, Flow Cytmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human VISTA aa 250-311 (C terminal). The exact sequence is proprietary. NP_071436.1 and Gene ID 64115.
Database link: Q9H7M9 -
ポジティブ・コントロール
- IP: Jurkat WB: Jurkat, MOLT-4, MJ, RPMI 8226, HeLa, KG-1 IHC-P: Human lung carcinoma, ICC: NCI H226 Flow Cyt: human peripheral blood mononuclear cells
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特記事項
This product is sold under License from Bethyl Laboratories, Inc.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading...
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特記事項(精製)
Recombinant antibody was purified from cell culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
BLR035F -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab243891の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000.
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ICC |
1/100 - 1/500.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at an assay dependent concentration.
Use 20µl/mg lysate. |
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Flow Cyt |
Use 2µl for 106 cells.
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特記事項 |
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WB
1/1000. |
ICC
1/100 - 1/500. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. Use 20µl/mg lysate. |
Flow Cyt
Use 2µl for 106 cells. |
ターゲット情報
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配列類似性
Contains 1 Ig-like (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 64115 Human
- Omim: 615608 Human
- SwissProt: Q9H7M9 Human
- Unigene: 47382 Human
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別名
- B7 H5 antibody
- B7H5 antibody
- C10orf54 antibody
see all
画像
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VISTA was immunoprecipitated from 1.0 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab243891 at 20 µl per reaction. Western blot was performed on the immunoprecipitate using ab243891 at 1/1000 dilution.
Lane 1: ab243891 IP in Jurkat whole cell lysate.
Lane 2: Contol IgG in Jurkat whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
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Western blot analysis using ab243891 at 1/1000 dilution.
Lane 1: Jurkat whole cell lysate (50 µg).
Lane 2: MOLT-4 whole cell lysate (50 µg).
Lane 3: MJ whole cell lysate (50 µg).
Lane 4: RPMI 8226 whole cell lysate (50 µg).
Lane 5: HeLa whole cell lysate (50 µg).
Lane 6: KG-1 whole cell lysate (50 µg).
Lane 7: HEK-293T whole cell lysate (50 µg).
Lane 8: SUP-M2 whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds.
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Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for VISTA using ab243891 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit antibody was used as the secondary. DAB staining.
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma tissue labeling VISTA with ab243891 at a 1/250 dilution, followed by HRP-conjugated Goat Anti-Rabit IgG secondary antibody. Substrate: Opal. Counterstain: DAPI (blue).
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Formalin-fixed, paraffin-embedded NCI H226 cells labeling VISTA using ab243891 at 1/250 dilution in ICC analysis. A HRP-conjugated goat-anti rabbit IgG was used as the secondary. DAB staining.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab243891 は 1 報の論文で使用されています。
- Schlichtner S et al. Functional role of galectin-9 in directing human innate immune reactions to Gram-negative bacteria and T cell apoptosis. Int Immunopharmacol 100:108155 (2021). PubMed: 34543981