Anti-Visfatin 抗体 [EPR21980] (ab236874)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21980] to Visfatin
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Visfatin antibody [EPR21980]
Visfatin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR21980] to Visfatin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)more details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: K562, Raji, HeLa, HT-1080, PC-3, MCF7, HCT 116, C6, PC-12 and Raw 264.7 whole cell lysate; Human fetal kidney lysate. IHC-P: Human bladder cancer and breast cancer tissue; Mouse kidney tissue; Rat liver tissue. ICC/IF: HCT 116 and K562 cells. Flow: K562 and HCT 116 cells. IP: K562 whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR21980 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab236874の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 55 kDa.
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). |
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ICC/IF |
1/100.
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IP |
1/30.
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Flow Cyt (Intra) |
1/500.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 55 kDa. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). |
ICC/IF
1/100. |
IP
1/30. |
Flow Cyt (Intra)
1/500. |
ターゲット情報
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機能
Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. -
組織特異性
Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues. -
パスウェイ
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1. -
配列類似性
Belongs to the NAPRTase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 10135 Human
- Entrez Gene: 59027 Mouse
- Entrez Gene: 297508 Rat
- Omim: 608764 Human
- SwissProt: P43490 Human
- SwissProt: Q99KQ4 Mouse
- SwissProt: Q80Z29 Rat
- Unigene: 489615 Human
see all -
別名
- 1110035O14Rik antibody
- AI314458 antibody
- AI480535 antibody
see all
画像
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All lanes : Anti-Visfatin antibody [EPR21980] (ab236874) at 1/1000 dilution
Lane 1 : K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg
Lane 2 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate at 20 µg
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 4 : HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate at 20 µg
Lane 5 : PC-3 (human prostate adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 6 : Human fetal kidney lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 55 kDaBlocking/Diluting buffer and concentration: 5% NFDM/TBST
Exposure times:
Lanes 1-2: 26 seconds
Lanes 3-5: 92 seconds
Lane 6: 3 minutesLane 6 was developed using a higher sensitivity ECL substrate.
The molecular weight observed is consistent with what has been described in the literature (PMID:25368373, PMID:12782293).
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Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling Visfatin using ab236874 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining in human bladder cancer (PMID:26396920) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cells labeling Visfatin (Green) with ab236874 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Confocal image showing cytoplasmic and nuclear staining in HCT 116 cell line is observed. The nuclear counterstain is DAPI (Blue). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as the counterstain (Red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Alexa-Fluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
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Visfatin was immunoprecipitated from 0.35 mg of K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab236874 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab236874 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: K562 whole cell lysate 10 μg (input).
Lane 2: ab236874 IP in K562 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236874 in K562 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds. -
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cell line labeling Visfatin with ab236874 at 1/500 dilution (Red) compared with the Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody, Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized K562 (human chronic myelogenous leukemia lymphoblast) cell line labeling Visfatin with ab236874 at 1/500 dilution (Red) compared with the Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody, Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Visfatin using ab236874 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining in rat liver (PMID: 29104634; PMID:24603648) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Visfatin using ab236874 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining in mouse kidney (PMID: 29104634; PMID:24287278) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Visfatin using ab236874 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear and cytoplasmic staining in human breast cancer (PMID:21784959) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Visfatin antibody [EPR21980] (ab236874) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Lane 2 : HCT 116 (human colorectal carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 3 : HT-1080 (human fibrosarcoma epithelial cell), whole cell lysate at 20 µg
Lane 4 : C6 (rat glial tumor glial cell), whole cell lysate at 20 µg
Lane 5 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg
Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 55 kDaBlocking/Diluting buffer and concentration: 5% NFDM/TBST
Exposure time:
Lanes 1-3: 40 seconds
Lanes 4-5: 3 minutes
Lane 6: 15 secondsThe molecular weight observed is consistent with what has been described in the literature (PMID:25368373, PMID:12782293).
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized K562 (human chronic myelogenous leukemia lymphoblast) cells labeling Visfatin (Green) with ab236874 at 1/100 dilution, followed by AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Confocal image showing cytoplasmic and nuclear staining in K562 cell line is observed. The nuclear counterstain is DAPI (Blue). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used as the counterstain (Red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Alexa-Fluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (11)
ab236874 は 11 報の論文で使用されています。
- Zhuan Q et al. Nampt affects mitochondrial function in aged oocytes by mediating the downstream effector FoxO3a. J Cell Physiol 237:647-659 (2022). PubMed: 34318928
- Higgins CB et al. SIRT1 selectively exerts the metabolic protective effects of hepatocyte nicotinamide phosphoribosyltransferase. Nat Commun 13:1074 (2022). PubMed: 35228549
- Deng Y et al. A Nicotinamide Phosphoribosyltransferase Inhibitor, FK866, Suppresses the Growth of Anaplastic Meningiomas and Inhibits Immune Checkpoint Expression by Regulating STAT1. Front Oncol 12:836257 (2022). PubMed: 35515130
- Kuang ZS et al. Inhibition of visfatin alleviates sepsis-induced intestinal damage by inhibiting Hippo signaling pathway. Inflamm Res 71:911-922 (2022). PubMed: 35731253
- Munuera-Cabeza M et al. Pantothenate and L-Carnitine Supplementation Improves Pathological Alterations in Cellular Models of KAT6A Syndrome. Genes (Basel) 13:N/A (2022). PubMed: 36553567