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  1. Link

    vinculin-antibody-vin-54-ab130007.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Actin Binding Proteins
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Anti-Vinculin 抗体 [VIN-54] (ab130007)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (11)

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Western blot - Anti-Vinculin antibody [VIN-54] (ab130007)
  • Immunohistochemistry (Frozen sections) - Anti-Vinculin antibody [VIN-54] (ab130007)
  • Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [VIN-54] (ab130007)
  • Immunohistochemistry (Frozen sections) - Anti-Vinculin antibody [VIN-54] (ab130007)
  • Western blot - Anti-Vinculin antibody [VIN-54] (ab130007)

Key features and details

  • Mouse monoclonal [VIN-54] to Vinculin
  • Suitable for: ICC/IF, WB, IHC-Fr
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG1

こちらの製品もご検討ください

一次抗体
Product image
Anti-Vinculin antibody [EPR8185] (ab129002)
タンパク質
Product image
Recombinant Human Vinculin protein (ab131747)
一次抗体
Product image
Anti-beta I Tubulin antibody [2A1A9] (ab204947)

関連製品

製品の概要

  • 製品名

    Anti-Vinculin antibody [VIN-54]
    Vinculin 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [VIN-54] to Vinculin
  • 由来種

    Mouse
  • アプリケーション

    適用あり: ICC/IF, WB, IHC-Frmore details
  • 種交差性

    交差種: Mouse, Rat, Human, African green monkey
  • 免疫原

    Human Vinculin purified from uterus.

  • ポジティブ・コントロール

    • WB: Hela, HepG2, Cos-7, U-87 MG, PC-12, RH35, HEPA1-6 and NIH-3T3 whole cell lysates; ICC/IF: Human mammary cancer cells; IHC-P: Human breast tissue.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7.2
    Preservative: 0.001% Sodium azide
    Constituents: 1.2% Sodium acetate, 0.2% BSA
  • Concentration information loading...
  • 精製度

    Protein G purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    VIN-54
  • アイソタイプ

    IgG1
  • 研究分野

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Binding Proteins
    • Cardiovascular
    • Heart
    • Cardiac arrhythmias
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Actins
    • Isotype/Loading Controls
    • Loading Controls
    • Vinculin

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Vinculin protein (ab131747)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab130007の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 2 µg/ml.
WB (4)
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 124 kDa.
IHC-Fr
Use a concentration of 2 - 4 µg/ml.
特記事項
ICC/IF
Use a concentration of 2 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 124 kDa.
IHC-Fr
Use a concentration of 2 - 4 µg/ml.

ターゲット情報

  • 機能

    Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.
  • 組織特異性

    Metavinculin is muscle-specific.
  • 関連疾患

    Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) [MIM:611407]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in VCL are the cause of cardiomyopathy familial hypertrophic type 15 (CMH15) [MIM:613255]. It is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
  • 配列類似性

    Belongs to the vinculin/alpha-catenin family.
  • ドメイン

    Exists in at least two conformations. When in the closed, 'inactive' conformation, extensive interactions between the head and tail domains prevent detectable binding to most of its ligands. It takes on an 'active' conformation after cooperative and simultaneous binding of two different ligands. This activation involves displacement of the head-tail interactions and leads to a significant accumulation of ternary complexes. The active form then binds a number of proteins that have both signaling and structural roles that are essential for cell adhesion.
    The N-terminal globular head (Vh) comprises of subdomains D1-D4. The C-terminal tail (Vt) binds F-actin and cross-links actin filaments into bundles. An intramolecular interaction between Vh and Vt masks the F-actin-binding domain located in Vt. The binding of talin and alpha-actinin to the D1 subdomain of vinculin induces a helical bundle conversion of this subdomain, leading to the disruption of the intramolecular interaction and the exposure of the cryptic F-actin-binding domain of Vt. Vt inhibits actin filament barbed end elongation without affecting the critical concentration of actin assembly.
  • 翻訳後修飾

    Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques.
    Aceylated; mainly by myristic acid but also small amount of palmitic acid.
  • 細胞内局在

    Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions.
  • Target information above from: UniProt accession P18206 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 7414 Human
    • Entrez Gene: 22330 Mouse
    • Entrez Gene: 305679 Rat
    • Omim: 193065 Human
    • SwissProt: P18206 Human
    • SwissProt: Q64727 Mouse
    • SwissProt: P85972 Rat
    • Unigene: 643896 Human
    • Unigene: 279361 Mouse
    • Unigene: 164613 Rat
    see all
  • 別名

    • CMD1W antibody
    • CMH15 antibody
    • Epididymis luminal protein 114 antibody
    • HEL114 antibody
    • Metavinculin antibody
    • MV antibody
    • MVCL antibody
    • OTTHUMP00000019861 antibody
    • OTTHUMP00000019862 antibody
    • VCL antibody
    • VINC antibody
    • VINC_HUMAN antibody
    • Vinculin antibody
    see all

画像

  • Western blot - Anti-Vinculin antibody [VIN-54] (ab130007)
    Western blot - Anti-Vinculin antibody [VIN-54] (ab130007)
    All lanes : Anti-Vinculin antibody [VIN-54] (ab130007) at 0.5 µg/ml

    Lane 1 : Hela whole cell lysates
    Lane 2 : HepG2 whole cell lysates
    Lane 3 : Monkey COS-7 whole cell lysates
    Lane 4 : U-87MG whole cell lysates
    Lane 5 : Rat PC-12 whole cell lysates
    Lane 6 : Rat RH35 whole cell lysates
    Lane 7 : Mouse HEPA1-6 whole cell lysates
    Lane 8 : Mouse NIH3T3 whole cell lysates

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat anti- mouse IgG-HRP at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 124 kDa
    Observed band size: 124 kDa



    The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.

    After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT.

     

     

     

  • Immunohistochemistry (Frozen sections) - Anti-Vinculin antibody [VIN-54] (ab130007)
    Immunohistochemistry (Frozen sections) - Anti-Vinculin antibody [VIN-54] (ab130007)

    Immunohistochemical analysis of frozen sections of human placenta tissue labelling Vinculin with ab130007 at 1μg/ml, followed by a biotinylated goat anti-mouse IgG secondary antibody. The tissue was blocked with 10% goat serum. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

  • Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [VIN-54] (ab130007)
    Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [VIN-54] (ab130007)

    Immunocytochemistry/Immunofluorescent analysis of human mammary cancer cells labelling Vinculin with ab130007 at 2μg/ml, followed by a DyLight®488 Conjugated Goat Anti-Mouse secondary antibody at 1/100 dilution. DAPI was used as a nuclear counterstain.

     

  • Immunohistochemistry (Frozen sections) - Anti-Vinculin antibody [VIN-54] (ab130007)
    Immunohistochemistry (Frozen sections) - Anti-Vinculin antibody [VIN-54] (ab130007)

    Immunohistochemical analysis of frozen sections of human placenta tissue labelling Vinculin with ab130007 at 1μg/ml, followed by a biotinylated goat anti-mouse IgG secondary antibody. The tissue was blocked with 10% goat serum. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

  • Western blot - Anti-Vinculin antibody [VIN-54] (ab130007)
    Western blot - Anti-Vinculin antibody [VIN-54] (ab130007)
    All lanes : Anti-Vinculin antibody [VIN-54] (ab130007) at 1/10000 dilution

    Lane 1 : HAP1
    Lane 2 : HEK293
    Lane 3 : U-2 OS

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 124 kDa



    ab130007 was shown to recognize vinculin when whole cell lysates were subjected to SDS-PAGE. The blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes which was incubated overnight at 4°C at 0.1 μg antibody per mL and developed with Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibody at 1/15000 dilution for 1 hour at room temperature before imaging.

プロトコール

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (11)

ab130007 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab130007 は 11 報の論文で使用されています。

  • Johari M  et al. Missense mutations in small muscle protein X-linked (SMPX) cause distal myopathy with protein inclusions. Acta Neuropathol 142:375-393 (2021). PubMed: 33974137
  • Fox JL  et al. Cryo-EM structural analysis of FADD:Caspase-8 complexes defines the catalytic dimer architecture for co-ordinated control of cell fate. Nat Commun 12:819 (2021). PubMed: 33547302
  • Delgado C  et al. Neural cell adhesion molecule is required for ventricular conduction system development. Development 148:N/A (2021). PubMed: 34100064
  • Wu F  et al. The Oncogenic Role of APC/C Activator Protein Cdc20 by an Integrated Pan-Cancer Analysis in Human Tumors. Front Oncol 11:721797 (2021). PubMed: 34527589
  • Pillai-Kastoori L  et al. Antibody validation for Western blot: By the user, for the user. J Biol Chem 295:926-939 (2020). PubMed: 31819006
View all Publications for this product

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1-5 of 5 Abreviews or Q&A

Western blot abreview for Anti-Vinculin antibody [VIN-54]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
90 µg
Specification
Cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Abcam user community

Verified customer

投稿 Nov 24 2021

Western blot abreview for Anti-Vinculin antibody [VIN-54]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
60 µg
Specification
cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Abcam user community

Verified customer

投稿 Nov 16 2021

Western blot abreview for Anti-Vinculin antibody [VIN-54]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
90 µg
Specification
Cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Abcam user community

Verified customer

投稿 Oct 11 2021

Western blot abreview for Anti-Vinculin antibody [VIN-54]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cell line)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
70 µg
Treatment
5uM MG132 for 6 hrs
Specification
Cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

投稿 Sep 07 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vinculin antibody [VIN-54]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (184A1 mammary epithelial)
Permeabilization
Yes - PBS + 0.25% Triton-X
Specification
184A1 mammary epithelial
Blocking step
LI-COR Intercept Blocking Buffeer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Sep 03 2020

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