Anti-Vinculin 抗体 [VIN-54] (ab130007)
![Western blot - Anti-Vinculin antibody [VIN-54] (ab130007)](https://www.abcam.co.jp/ps/products/130/ab130007/Images/ab130007-356277-anti-vinculin-antibody-vin-54-western-blot-human.JPG "Western blot - Anti-Vinculin antibody [VIN-54] (ab130007)")
Key features and details
- Mouse monoclonal [VIN-54] to Vinculin
- Suitable for: ICC/IF, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
製品の概要
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製品名
Anti-Vinculin antibody [VIN-54]
Vinculin 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [VIN-54] to Vinculin -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WB, IHC-Frmore details -
種交差性
交差種: Mouse, Rat, Human, African green monkey -
免疫原
Human Vinculin purified from uterus.
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ポジティブ･コントロール
- WB: Hela, HepG2, Cos-7, U-87 MG, PC-12, RH35, HEPA1-6 and NIH-3T3 whole cell lysates; ICC/IF: Human mammary cancer cells; IHC-P: Human breast tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.001% Sodium azide
Constituents: 1.2% Sodium acetate, 0.2% BSA -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
VIN-54 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab130007の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 2 µg/ml.
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| WB | (4) |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 124 kDa.
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| IHC-Fr |
Use a concentration of 2 - 4 µg/ml.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 2 µg/ml. |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 124 kDa. |
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IHC-Fr
Use a concentration of 2 - 4 µg/ml. |
ターゲット情報
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機能
Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. -
組織特異性
Metavinculin is muscle-specific. -
関連疾患
Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) [MIM:611407]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in VCL are the cause of cardiomyopathy familial hypertrophic type 15 (CMH15) [MIM:613255]. It is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death. -
配列類似性
Belongs to the vinculin/alpha-catenin family. -
ドメイン
Exists in at least two conformations. When in the closed, 'inactive' conformation, extensive interactions between the head and tail domains prevent detectable binding to most of its ligands. It takes on an 'active' conformation after cooperative and simultaneous binding of two different ligands. This activation involves displacement of the head-tail interactions and leads to a significant accumulation of ternary complexes. The active form then binds a number of proteins that have both signaling and structural roles that are essential for cell adhesion.
The N-terminal globular head (Vh) comprises of subdomains D1-D4. The C-terminal tail (Vt) binds F-actin and cross-links actin filaments into bundles. An intramolecular interaction between Vh and Vt masks the F-actin-binding domain located in Vt. The binding of talin and alpha-actinin to the D1 subdomain of vinculin induces a helical bundle conversion of this subdomain, leading to the disruption of the intramolecular interaction and the exposure of the cryptic F-actin-binding domain of Vt. Vt inhibits actin filament barbed end elongation without affecting the critical concentration of actin assembly. -
翻訳後修飾
Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques.
Aceylated; mainly by myristic acid but also small amount of palmitic acid. -
細胞内局在
Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. - Information by UniProt
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参照データベース
- Entrez Gene: 7414 Human
- Entrez Gene: 22330 Mouse
- Entrez Gene: 305679 Rat
- Omim: 193065 Human
- SwissProt: P18206 Human
- SwissProt: Q64727 Mouse
- SwissProt: P85972 Rat
- Unigene: 643896 Human
see all -
別名
- CMD1W antibody
- CMH15 antibody
- Epididymis luminal protein 114 antibody
see all
画像
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Western blot - Anti-Vinculin antibody [VIN-54] (ab130007)All lanes : Anti-Vinculin antibody [VIN-54] (ab130007) at 0.5 µg/ml
Lane 1 : Hela whole cell lysates
Lane 2 : HepG2 whole cell lysates
Lane 3 : Monkey COS-7 whole cell lysates
Lane 4 : U-87MG whole cell lysates
Lane 5 : Rat PC-12 whole cell lysates
Lane 6 : Rat RH35 whole cell lysates
Lane 7 : Mouse HEPA1-6 whole cell lysates
Lane 8 : Mouse NIH3T3 whole cell lysates
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat anti- mouse IgG-HRP at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 124 kDa
Observed band size: 124 kDaThe signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT.
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![Immunohistochemistry (Frozen sections) - Anti-Vinculin antibody [VIN-54] (ab130007)](https://www.abcam.co.jp/ps/products/130/ab130007/Images/ab130007-356276-anti-vinculin-antibody-vin-54-immunohistochemistry-human-placenta.JPG)
Immunohistochemistry (Frozen sections) - Anti-Vinculin antibody [VIN-54] (ab130007)Immunohistochemical analysis of frozen sections of human placenta tissue labelling Vinculin with ab130007 at 1μg/ml, followed by a biotinylated goat anti-mouse IgG secondary antibody. The tissue was blocked with 10% goat serum. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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![Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [VIN-54] (ab130007)](https://www.abcam.co.jp/ps/products/130/ab130007/Images/ab130007-356278-anti-vinculin-antibody-vin-54-immunocytochemistry-immunofluorescence-mammary-gland-cancer-human.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-Vinculin antibody [VIN-54] (ab130007)Immunocytochemistry/Immunofluorescent analysis of human mammary cancer cells labelling Vinculin with ab130007 at 2μg/ml, followed by a DyLight®488 Conjugated Goat Anti-Mouse secondary antibody at 1/100 dilution. DAPI was used as a nuclear counterstain.
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![Immunohistochemistry (Frozen sections) - Anti-Vinculin antibody [VIN-54] (ab130007)](https://www.abcam.co.jp/ps/products/130/ab130007/Images/ab130007-356275-anti-vinculin-antibody-vin-54-immunohistochemistry-placenta-human.JPG)
Immunohistochemistry (Frozen sections) - Anti-Vinculin antibody [VIN-54] (ab130007)Immunohistochemical analysis of frozen sections of human placenta tissue labelling Vinculin with ab130007 at 1μg/ml, followed by a biotinylated goat anti-mouse IgG secondary antibody. The tissue was blocked with 10% goat serum. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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![Western blot - Anti-Vinculin antibody [VIN-54] (ab130007)](https://www.abcam.co.jp/ps/products/130/ab130007/Images/ab130007-286744-anti-vinculin-antibody-vin-54-western-blot.jpg)
Western blot - Anti-Vinculin antibody [VIN-54] (ab130007)All lanes : Anti-Vinculin antibody [VIN-54] (ab130007) at 1/10000 dilution
Lane 1 : HAP1
Lane 2 : HEK293
Lane 3 : U-2 OS
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 124 kDaab130007 was shown to recognize vinculin when whole cell lysates were subjected to SDS-PAGE. The blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes which was incubated overnight at 4°C at 0.1 μg antibody per mL and developed with Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibody at 1/15000 dilution for 1 hour at room temperature before imaging.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab130007 は 11 報の論文で使用されています。
- Johari M et al. Missense mutations in small muscle protein X-linked (SMPX) cause distal myopathy with protein inclusions. Acta Neuropathol 142:375-393 (2021). PubMed: 33974137
- Fox JL et al. Cryo-EM structural analysis of FADD:Caspase-8 complexes defines the catalytic dimer architecture for co-ordinated control of cell fate. Nat Commun 12:819 (2021). PubMed: 33547302
- Delgado C et al. Neural cell adhesion molecule is required for ventricular conduction system development. Development 148:N/A (2021). PubMed: 34100064
- Wu F et al. The Oncogenic Role of APC/C Activator Protein Cdc20 by an Integrated Pan-Cancer Analysis in Human Tumors. Front Oncol 11:721797 (2021). PubMed: 34527589
- Pillai-Kastoori L et al. Antibody validation for Western blot: By the user, for the user. J Biol Chem 295:926-939 (2020). PubMed: 31819006