Anti-Vinculin 抗体 [VCL/2575] - BSA and Azide free (ab269690)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (ab269690)
  • Western blot - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (ab269690)
  • Protein Array - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (ab269690)

Key features and details

  • Mouse monoclonal [VCL/2575] to Vinculin - BSA and Azide free
  • Suitable for: WB, IHC-P, Protein Array
  • Reacts with: Mouse, Human
  • Isotype: IgG2b

製品の概要

  • 製品名

    Anti-Vinculin antibody [VCL/2575] - BSA and Azide free
    Vinculin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [VCL/2575] to Vinculin - BSA and Azide free

  • 由来種

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment within Human Vinculin aa 174-322. The exact sequence is proprietary.
    Database link: P18206

  • ポジティブ･コントロール

    • WB: A431, K562 and NIH/3T3 cell lysate. IHC-P: Human testicular carcinoma tissue.

  • 特記事項

    ab269690 is the PBS only version of ab269680.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-P
Human
WB
Mouse
Human
アプリケーション Abreviews 特記事項
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 124 kDa.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protein Array
Use at an assay dependent concentration.
特記事項
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 124 kDa.
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protein Array
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion.

  • 組織特異性

    Metavinculin is muscle-specific.

  • 関連疾患

    Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) [MIM:611407]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in VCL are the cause of cardiomyopathy familial hypertrophic type 15 (CMH15) [MIM:613255]. It is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.

  • 配列類似性

    Belongs to the vinculin/alpha-catenin family.

  • ドメイン

    Exists in at least two conformations. When in the closed, 'inactive' conformation, extensive interactions between the head and tail domains prevent detectable binding to most of its ligands. It takes on an 'active' conformation after cooperative and simultaneous binding of two different ligands. This activation involves displacement of the head-tail interactions and leads to a significant accumulation of ternary complexes. The active form then binds a number of proteins that have both signaling and structural roles that are essential for cell adhesion.
    The N-terminal globular head (Vh) comprises of subdomains D1-D4. The C-terminal tail (Vt) binds F-actin and cross-links actin filaments into bundles. An intramolecular interaction between Vh and Vt masks the F-actin-binding domain located in Vt. The binding of talin and alpha-actinin to the D1 subdomain of vinculin induces a helical bundle conversion of this subdomain, leading to the disruption of the intramolecular interaction and the exposure of the cryptic F-actin-binding domain of Vt. Vt inhibits actin filament barbed end elongation without affecting the critical concentration of actin assembly.

  • 翻訳後修飾

    Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques.
    Aceylated; mainly by myristic acid but also small amount of palmitic acid.

  • 細胞内局在

    Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell membrane. Cytoplasmic face of adhesion plaques. Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CMD1W antibody
      • CMH15 antibody
      • Epididymis luminal protein 114 antibody
      • HEL114 antibody
      • Metavinculin antibody
      • MV antibody
      • MVCL antibody
      • OTTHUMP00000019861 antibody
      • OTTHUMP00000019862 antibody
      • VCL antibody
      • VINC antibody
      • VINC_HUMAN antibody
      • Vinculin antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (ab269690)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (ab269690)

      Formalin/PFA-fixed paraffin-embedded human testicular carcinoma tissue stained for Vinculin using ab269680 at 2 µg/ml in immunohistochemistry.

      This image was produced using the same antibody clone but in a different formulation containing PBS, BSA and Sodium Azide (ab269680).

    • Western blot - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (ab269690)
      Western blot - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (ab269690)
      All lanes : Anti-Vinculin antibody [VCL/2575] (ab269680) at 2 µg/ml

      Lane 1 : A431 (Human epidermoid carcinoma cell line) cell lysate
      Lane 2 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) cell lysate
      Lane 3 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

      Predicted band size: 124 kDa



      This image was produced using the same antibody clone but in a different formulation containing PBS, BSA and Sodium Azide (ab269680).

    • Protein Array - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (ab269690)
      Protein Array - Anti-Vinculin antibody [VCL/2575] - BSA and Azide free (ab269690)

      Analysis of Protein Array containing more than 19,000 full-length human proteins using ab269680.
      - and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

      This image was produced using the same antibody clone but in a different formulation containing PBS, BSA and Sodium Azide (ab269680).

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab269690 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab269690 は論文での使用が確認できていません。

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