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リコンビナントRabMAb

Anti-Vimentin (phospho S56) 抗体 [EPR21084] (ab217673)

Submit a review Submit a question References (4)
Western blot - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)
  • Flow Cytometry (Intracellular) - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)
  • Dot Blot - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)
  • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)
  • Immunoprecipitation - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)
  • Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21084] to Vimentin (phospho S56)
  • Suitable for: WB, Dot blot, ICC/IF, IP, Flow Cyt (Intra)
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Vimentin (phospho S56) antibody [EPR21084]
    Vimentin 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR21084] to Vimentin (phospho S56)

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, Dot blot, ICC/IF, IP, Flow Cyt (Intra)more details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate. ICC/IF: HeLa cells. IP: HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab217673の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 54 kDa).
Dot blot
1/1000.
ICC/IF
1/1000.
IP
1/30.
Flow Cyt (Intra)
Use at an assay dependent concentration.
特記事項
WB
1/1000. Detects a band of approximately 57 kDa (predicted molecular weight: 54 kDa).
Dot blot
1/1000.
ICC/IF
1/1000.
IP
1/30.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

  • 組織特異性

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.

  • 関連疾患

    Cataract 30

  • 配列類似性

    Belongs to the intermediate filament family.

  • ドメイン

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.

  • 翻訳後修飾

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 製品の状態

      Vimentin is found in connective tissue and in the cytoskeleton.

    • 別名

      • CTRCT30 antibody
      • Epididymis luminal protein 113 antibody
      • FLJ36605 antibody
      • HEL113 antibody
      • VIM antibody
      • VIME_HUMAN antibody
      • Vimentin antibody
      see all

    画像

    • Western blot - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)
      Western blot - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)
      All lanes : Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673) at 1/1000 dilution

      Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Developed using the ECL technique.

      Predicted band size: 54 kDa
      Observed band size: 57 kDa why is the actual band size different from the predicted?


      Exposure time: 23 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)
      Flow Cytometry (Intracellular) - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)

      Ab217673 staining Vimentin (phospho S56) in HeLa (human cervix adenocarcinoma epithelial cell) cells by intracellular flow cytometry. Cells were fixed using 80% Methanol and permeabilized with 0.1% Tween-20. The sample was incubated with primary antibody at 1/500 dilution. An Alexa Fluor®488 Goat anti-rabbit IgG (ab150077) was used as a secondary antibody. Rabbit monoclonal IgG (ab172730) was used as an isotype control (left). Cells were pre-treated with 20 µg/ml RNase A for 30 minutes to eliminate the non-specific binding between RNA and PI (Propidium iodide). Vimentiin (phospho S56) is highly expressed in mitotic cells. (PMID: 16260496) 

       

       

    • Dot Blot - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)
      Dot Blot - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)

      Dot blot analysis of Vimentin (phospho S56) labeled with ab217673 at 1/1000 dilution.

      Lane 1: Vimentin (phospho S56) peptide.
      Lane 2: Vimentin non-phospho peptide.

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.

      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 1 minute.

      The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

    • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)
      Immunocytochemistry/ Immunofluorescence - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Vimentin (phospho S56) with ab217673 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing strong positive staining in HeLa cells in M phase.

      The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

      Secondary antibody only control: PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Immunoprecipitation - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)
      Immunoprecipitation - Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)

      Vimentin (phospho S56) was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate with ab217673 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab217673 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

      Lane 1: HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate 10 μg (Input).
      Lane 2: ab217673 IP in HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab217673 in HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)
      Anti-Vimentin (phospho S56) antibody [EPR21084] (ab217673)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (4)

    ab217673 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab217673 は 4 報の論文で使用されています。

    • Li Y  et al. Upregulation of SIX4 indicates poor clinical outcome and promotes tumor growth and cell metastasis in esophageal squamous cell carcinoma. Thorac Cancer 12:752-759 (2021). PubMed: 33481352
    • Sun L  et al. MicroRNA-615 functions as a tumor suppressor in osteosarcoma through the suppression of HK2. Oncol Lett 20:226 (2020). PubMed: 32968448
    • Chen W  et al. TERF1 downregulation promotes the migration and invasion of the PC3 prostate cancer cell line as a target of miR-155. Mol Med Rep 22:5209-5218 (2020). PubMed: 33174061
    • Liu J  et al. Bnip3 interacts with vimentin, an intermediate filament protein, and regulates autophagy of hepatic stellate cells. Aging (Albany NY) 13:957-972 (2020). PubMed: 33290258

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