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    vimentin-antibody-vi-10-ab20346.pdf

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遺伝子ノックアウト細胞株 検証済

Anti-Vimentin 抗体 [VI-10] (ab20346)

  • Datasheet
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Reviews (20)Q&A (3)References (89)

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Western blot - Anti-Vimentin antibody [VI-10] (ab20346)
  • Immunocytochemistry - Anti-Vimentin antibody [VI-10] (ab20346)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-10] (ab20346)
  • Immunocytochemistry - Anti-Vimentin antibody [VI-10] (ab20346)
  • Immunocytochemistry - Anti-Vimentin antibody [VI-10] (ab20346)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-10] (ab20346)
  • Western blot - Anti-Vimentin antibody [VI-10] (ab20346)
  • Flow Cytometry - Anti-Vimentin antibody [VI-10] (ab20346)
  • Immunocytochemistry - Anti-Vimentin antibody [VI-10] (ab20346)

Key features and details

  • Mouse monoclonal [VI-10] to Vimentin
  • Suitable for: WB, IHC-P, Flow Cyt, ICC
  • Knockout validated
  • Reacts with: Mouse, Rat, Chicken, Human
  • Isotype: IgM

こちらの製品もご検討ください

一次抗体
Product image
FITC Anti-Vimentin antibody [RV202] (ab128507)
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FITC Anti-CD3 antibody [CD3-12] (ab34722)
一次抗体
Product image
Anti-Cytokeratin 7 antibody [EPR1619Y] - Cytoskeleton Marker (ab68459)

関連製品

製品の概要

  • 製品名

    Anti-Vimentin antibody [VI-10]
    Vimentin 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [VI-10] to Vimentin
  • 由来種

    Mouse
  • 特異性

    The antibody VI-10 reacts with vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types.
  • アプリケーション

    適用あり: WB, IHC-P, Flow Cyt, ICCmore details
  • 種交差性

    交差種: Mouse, Rat, Chicken, Human
    交差が予測される動物種: Pig
  • 免疫原

    Full length native protein (purified) corresponding to Vimentin.

  • ポジティブ・コントロール

    • ウェスタン・ブロット用ポジティブコントロールRecombinant Human Vimentin protein
    • ICC: HAP1 cells; Mouse dissociated neural precursor cells; Chicken postnatal erythrocytes; RBL rat basophiilioc cells. IHC-P: Human skin tissue. WB: HAP1 cell lysate. Flow Cyt: NIH3T3 cells.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    pH: 8.0
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Proprietary Purification
  • 特記事項(精製)

    Purified by precipitation and chromatography.
  • ポリ/モノ

    モノクローナル
  • クローン名

    VI-10
  • ミエローマ

    unknown
  • アイソタイプ

    IgM
  • 軽鎖の種類

    unknown
  • 研究分野

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class III
    • Vimentin
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cancer
    • Tumor biomarkers
    • Other
    • Neuroscience
    • Neurology process
    • Neuroregeneration
    • Neuroregeneration
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (ab8516)
    • Rabbit Anti-Mouse IgG+IgM+IgA H&L (FITC) (ab8517)
    • Goat Anti-Mouse IgM H&L (ab9167)
    • Rabbit Anti-Mouse IgM mu chain (ab9175)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgM [B11/7] - Isotype control (ab91545)
  • Related Products

    • Human Vimentin Profiling ELISA Kit (ab173190)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab20346の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (3)
Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
IHC-P (4)
Use a concentration of 1 - 5 µg/ml.
Flow Cyt (1)
Use 1µg for 106 cells.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

ICC (2)
Use a concentration of 1 µg/ml.

Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C.

特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
IHC-P
Use a concentration of 1 - 5 µg/ml.
Flow Cyt
Use 1µg for 106 cells.

ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

ICC
Use a concentration of 1 µg/ml.

Staining technique: (a) fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C.

ターゲット情報

  • 機能

    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 組織特異性

    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • 関連疾患

    Cataract 30
  • 配列類似性

    Belongs to the intermediate filament family.
  • ドメイン

    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • 翻訳後修飾

    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • 細胞内局在

    Cytoplasm.
  • Target information above from: UniProt accession P08670 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 420519 Chicken
    • Entrez Gene: 7431 Human
    • Entrez Gene: 22352 Mouse
    • Entrez Gene: 100522394 Pig
    • Entrez Gene: 81818 Rat
    • Omim: 193060 Human
    • SwissProt: P09654 Chicken
    • SwissProt: P08670 Human
    • SwissProt: P20152 Mouse
    • SwissProt: P02543 Pig
    • SwissProt: P31000 Rat
    • Unigene: 455493 Human
    • Unigene: 691131 Human
    • Unigene: 268000 Mouse
    • Unigene: 2710 Rat
    see all
  • 製品の状態

    Vimentin is found in connective tissue and in the cytoskeleton.
  • 別名

    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

画像

  • Western blot - Anti-Vimentin antibody [VI-10] (ab20346)
    Western blot - Anti-Vimentin antibody [VI-10] (ab20346)
    All lanes : Anti-Vimentin antibody [VI-10] (ab20346) at 1 µg/ml

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : VIM (Vimentin) knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 54 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab20346 observed at 57 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab20346 was shown to specifically react with Vimentin in wild-type HAP1 cells as signal was lost in VIM (Vimentin) knockout cells. Wild-type and VIM (Vimentin) knockout samples were subjected to SDS-PAGE. Ab20346 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1 μg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry - Anti-Vimentin antibody [VI-10] (ab20346)
    Immunocytochemistry - Anti-Vimentin antibody [VI-10] (ab20346)

    ab20346 staining Vimentin in wild-type HAP1 cells (top panel) and Vimentin knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab20346 at 1μg/ml dilution and ab202272 at 1/250 dilution (shown in pseudo-color red) overnight at +4°C. The cells were then incubated with ab150117 (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)) at 1/1000 dilution for 1 hour. Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-10] (ab20346)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-10] (ab20346)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skin tissue labeling Vimentin with ab20346 at 5 ug/ml.

  • Immunocytochemistry - Anti-Vimentin antibody [VI-10] (ab20346)
    Immunocytochemistry - Anti-Vimentin antibody [VI-10] (ab20346)

    Immunocytochemical analysis of chicken postnatal erythrocytes labeling Vimentin with ab20346 at 1 ug/ml (green). Cells were counterstained with anti-alpha-tubulin (red) and DAPI (blue).

  • Immunocytochemistry - Anti-Vimentin antibody [VI-10] (ab20346)
    Immunocytochemistry - Anti-Vimentin antibody [VI-10] (ab20346)

    ab20346 at a 1/500 dilution staining mouse dissociated neural precursor cells by immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a goat anti-mouse Alexa-Fluor 568 (red stain) ab175473>ab175473). The image also shows a blue nuclear counterstain. Confocal image is shown with 2x zoom detail in top right corner.

    This image is courtesy of an Abreview submitted by Randal Moldich on 22 February 2006.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-10] (ab20346)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vimentin antibody [VI-10] (ab20346)

    Human normal skin. Staining is localised to cytoplasm. Left panel: with primary antibody at 1 ug/mL. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for mouse for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • Western blot - Anti-Vimentin antibody [VI-10] (ab20346)
    Western blot - Anti-Vimentin antibody [VI-10] (ab20346)Image from Gao MQ et al, J Cell Sci. 2010 Oct 15;123(Pt 20):3507-14. Epub 2010 Sep 14, Fig 3. DOI 10.1242/jcs.072900

    ab20346 used at a 1/1000 dilution in Western Blot.5-20µg of total protein from each sample was loaded.

  • Flow Cytometry - Anti-Vimentin antibody [VI-10] (ab20346)
    Flow Cytometry - Anti-Vimentin antibody [VI-10] (ab20346)

    Overlay histogram showing NIH3T3 cells stained with ab20346 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20346, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in NIH3T3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

  • Immunocytochemistry - Anti-Vimentin antibody [VI-10] (ab20346)
    Immunocytochemistry - Anti-Vimentin antibody [VI-10] (ab20346)

    Immunofluorescence staining of RBL rat basophiilioc cell line with anti-Vimentin (VI--10). Nuclei are stained with DAPI (blue).

     

プロトコール

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (89)

ab20346 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab20346 は 89 報の論文で使用されています。

  • Liu W  et al. miR-140 inhibits osteosarcoma progression by impairing USP22-mediated LSD1 stabilization and promoting p21 expression. Mol Ther Nucleic Acids 24:436-448 (2021). PubMed: 33868787
  • Bai Y  et al. Obacunone Attenuates Liver Fibrosis with Enhancing Anti-Oxidant Effects of GPx-4 and Inhibition of EMT. Molecules 26:N/A (2021). PubMed: 33435504
  • Fan Y  et al. CircNR3C2 promotes HRD1-mediated tumor-suppressive effect via sponging miR-513a-3p in triple-negative breast cancer. Mol Cancer 20:25 (2021). PubMed: 33530981
  • Feng Q  et al. Long non-coding RNA HOTAIR promotes the progression of synovial sarcoma through microRNA-126/stromal cell-derived factor-1 regulation. Oncol Lett 21:444 (2021). PubMed: 33868482
  • van Haaften EE  et al. Computationally guided in-vitro vascular growth model reveals causal link between flow oscillations and disorganized neotissue. Commun Biol 4:546 (2021). PubMed: 33972658
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 23 Abreviews or Q&A

Immunocytochemistry abreview for Anti-Vimentin antibody [VI-10]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Human Cultured Cells (Melanoma cells)
Permeabilization
No
Specification
Melanoma cells
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 4% · Temperature: 22°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jun 03 2021

Immunohistochemistry (Frozen sections) abreview for Anti-Vimentin antibody [VI-10]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Brain (Plexus))
Permeabilization
No
Specification
Brain (Plexus)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 10 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Vimentin antibody [VI-10]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Cortex (Brain))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ph = 6, citrate
Permeabilization
No
Specification
Cortex (Brain)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 26 2021

Immunohistochemistry (Frozen sections) abreview for Anti-Vimentin antibody [VI-10]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Choroid plexus)
Permeabilization
No
Specification
Choroid plexus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 26 2021

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Vimentin antibody [VI-10]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Melanoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Melanoma
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 17 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [VI-10]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Melanoma cells)
Permeabilization
No
Specification
Melanoma cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 21 2021

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Vimentin antibody [VI-10]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Human Tissue sections (Melanoma)
Antigen retrieval step
None
Permeabilization
No
Specification
Melanoma
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 21°C
Fixative
Acetone
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 17 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-Vimentin antibody [VI-10]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Sheep Cell (valve leaflet - tissue)
Permeabilization
Yes - Triton X-100
Specification
valve leaflet - tissue
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 19 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Vimentin antibody [VI-10]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 31 2018

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Vimentin antibody [VI-10]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate, pH 6
Specification
Prostate
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Kyle Wegner

Verified customer

投稿 Jan 02 2018

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