Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (colon fibroblast)
Permeabilization
Yes - 0.1% Triton
Specification
colon fibroblast
Blocking step
DAKO serum free protein blocker as blocking agent for 20 minute(s) · Concentration: 0.25% · Temperature: 28°C
Fixative
Methanol
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 28°C · Diluent: DAKO antibody diluent
Dilution
1/100
Additional data
Additional Notes
Nuclei were counterstained with propidium iodide.
PubMed ID
20592463: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. J Chai
Verified customer
投稿 Apr 13 2012