Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Synovial tissue)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: PBS/Protease
Permeabilization
No
Specification
Synovial tissue
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 20°C
Fixative
Formaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/BSA(2%)
Dilution
1/1000
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Biotinylated Goat Anti-Mouse IgG Antibody (Vector
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Abcam response
Thank you for your valuable feedback about the use of ab8978 in pig, a species we have not tested yet. We welcome any further feedback and AbReviews from other researchers who have used this antibody in pig which will help us assess how well the antibody works in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 09 2018