Application
Immunocytochemistry
Sample
Monodelphis domestica Cultured Cells (postnatal day(P)18 cortex)
Permeabilization
Yes - 0,1% Triton X-100 in PBS
Specification
postnatal day(P)18 cortex
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: O,5% BSA in PBS
Dilution
1/400
Secondary antibody
Dilution
1/300
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed
Additional data
Additional Notes
Primary neuronal cultures were derived from postnatal day (P)18 Monodelphis domestica (opossum) cortex and fixed at days in vitro (DIV)9. Scale bar, 50 um.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Jelena Ban
Verified customer
投稿 Apr 29 2021