Application
IHC - Wholemount
Sample
Mouse Embryo (E12.5 Mouse embryo)
Specification
E12.5 Mouse embryo
Other product details
Incubation time
99 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: PBS 1x +0.5% Gelatin +0.5% Triton
Dilution
1/500
Secondary antibody
Dilution
1/400
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed
Additional data
Additional Notes
Incubated for 7 days at 37°C in primary antibody
1-3 days in the secondary at 37°C
then cleared using iDisco+ protocol
Imaged with light sheet microscopy
Abcam response
We would like to thank the reviewer for submitting this AbReview. We are sorry to hear that this antibody did not work in your IHC-wholemount experiment using mouse embryo tissue Based on our internal testing this antibody does not work in IHC-wholemount and as such is not supported or approved by us for use in this application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Alain Chedotal
Verified customer
投稿 Feb 25 2021