Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Permeabilization
Yes - 0.2% Triton X100
Specification
NIH3T3
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
NIH3T3 cells fixed with 4% PFA for 20 mins. Nuclei were stained with H33342.
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投稿 Apr 05 2016