Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (12% page)
Sample
Human Cell lysate - whole cell (hep3b and L3.6PL)
Specification
hep3b and L3.6PL
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: skim milk 5%
Dilution
1/600
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit HRP , Jackson Labs
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/15000
Detection
Detection method
supersignal west pico, PIERCE
Bands
Specific: ~60 kDa Non-specific: Lower weight bands ( kDa
Exposure
10 minute(s) and 0 second(s)
Negative control
Hep3b is reported as a positive for Vimentin in WB
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投稿 Jan 16 2014