Anti-Vimentin 抗体 (ab137321)
Key features and details
- Rabbit polyclonal to Vimentin
- Suitable for: WB, ICC/IF, IHC-P, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Vimentin antibody
Vimentin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Vimentin -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-P, IPmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Guinea pig, Cow, Dog, Chimpanzee -
免疫原
Recombinant fragment within Human Vimentin (internal sequence). The exact sequence is proprietary.
Database link: P08670 -
ポジティブ・コントロール
- WB: HEK-293T, HeLa and NIH/3T3 whole cell lysates. U87-MG, SK-N-SH. IMR32 and SK-N-AS whole cell extracts. ICC/IF: HeLa cells. IHC-P: Adult mouse retina tissue. U373 xenograft tissue and RT2 rat xenograft tissue. IP: HeLa whole cell extract.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab137321の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 54 kDa.
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ICC/IF |
1/100 - 1/1000.
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IHC-P | (1) |
1/100 - 1/1000.
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IP |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 54 kDa. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. -
組織特異性
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. -
関連疾患
Cataract 30 -
配列類似性
Belongs to the intermediate filament family. -
ドメイン
The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. -
翻訳後修飾
Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 420519 Chicken
- Entrez Gene: 493952 Chimpanzee
- Entrez Gene: 280955 Cow
- Entrez Gene: 477991 Dog
- Entrez Gene: 100170760 Guinea pig
- Entrez Gene: 7431 Human
- Entrez Gene: 22352 Mouse
- Entrez Gene: 81818 Rat
see all -
製品の状態
Vimentin is found in connective tissue and in the cytoskeleton. -
別名
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
画像
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All lanes : Anti-Vimentin antibody (ab137321) at 1/50000 dilution
Lane 1 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell extracts
Lane 2 : SK-N-SH (Human neuroblastoma cell line) whole cell extracts
Lane 3 : IMR32 whole cell extracts
Lane 4 : SK-N-AS whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 54 kDa10% SDS-PAGE gel
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Paraffin-embedded adult mouse retina tissue stained for Vimentin with ab137321 (1/250 dilution) (green) in immunohistochemical analysis.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500.
Blue: Fluoroshield with DAPI. -
Paraffin-embedded rat testis tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse testis tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse ovary tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Vimentin (green) using ab137321 (1/500 dilution) in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstain: Hoechst 33342 (blue).
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Vimentin was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extracts using 5 µg of ab137321.
Lane 1: HeLa whole cell extracts (Input).
Lane 2: Control IgG in HeLa whole cell extracts.
Lane 3: Vimentin IP in HeLa whole cell extracts.
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Paraffin-embedded human lung carcinoma tissue stained for Vimentin with ab137321 at a 1/500 dilution in immunohistochemical analysis.
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Immunohistochemical analysis of paraffin-embedded, formalin-fixed RT2 rat xenograft tissue labeling Vimentin, using ab137321 at a 1/500 dilution.
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Anti-Vimentin antibody (ab137321) at 1/3000 dilution + HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg
Predicted band size: 54 kDa7.5% SDS PAGE
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Confocal immunofluorescence analysis of methanol-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Vimentin, using ab137321 (green) at a 1/500 dilution. Alpha-tubulin filaments were labelled with an anti-alpha tubilin antibody (red) at a 1/2000 dilution.
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All lanes : Anti-Vimentin antibody (ab137321) at 1/3000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 10 µg
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 5 µg
Predicted band size: 54 kDa10% SDS PAGE
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Anti-Vimentin antibody (ab137321) at 1/1000 dilution + NIH-3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 30 µg
Predicted band size: 54 kDa
10% SDS Page -
Immunohistochemical analysis of paraffin-embedded, formalin-fixed U373 xenograft tissue labeling Vimentin, using ab137321 at a 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (224)
ab137321 は 224 報の論文で使用されています。
- Zheng P et al. Circ_SATB2 knockdown inhibits the tumorigenesis of non-small cell lung cancer via miR-760/KIF2A axis. Histol Histopathol 38:431-441 (2023). PubMed: 36196919
- Tu J et al. PU.1 promotes development of rheumatoid arthritis via repressing FLT3 in macrophages and fibroblast-like synoviocytes. Ann Rheum Dis 82:198-211 (2023). PubMed: 36198439
- Ni Q et al. Exosomal microRNA-23a-3p contributes to the progression of cholangiocarcinoma by interaction with Dynamin3. Bioengineered 13:6208-6221 (2022). PubMed: 35200104
- Shi Y et al. NCAPG facilitates colorectal cancer cell proliferation, migration, invasion and epithelial-mesenchymal transition by activating the Wnt/β-catenin signaling pathway. Cancer Cell Int 22:119 (2022). PubMed: 35292013
- Wang Y et al. Circ_0005576 Exerts an Oncogenic Role in Cervical Cancer via miR-1305-Dependent Regulation of PAIP1. Reprod Sci 29:2647-2658 (2022). PubMed: 35378711