Anti-Villin 抗体 [EPR3490] (ab109516)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3490] to Villin
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Villin antibody [EPR3490]
Villin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3490] to Villin -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human small intestine, HT-29 and Caco-2 cell lysates; HT-29 cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3490 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109516の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 93 kDa).
|
特記事項 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Detects a band of approximately 95 kDa (predicted molecular weight: 93 kDa). |
ターゲット情報
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機能
Ca(2+)-regulated actin-binding protein. -
組織特異性
Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. -
配列類似性
Belongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.
Contains 1 HP (headpiece) domain. -
ドメイン
Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 7429 Human
- Entrez Gene: 22349 Mouse
- Entrez Gene: 316521 Rat
- Omim: 193040 Human
- SwissProt: P09327 Human
- SwissProt: Q62468 Mouse
- Unigene: 654595 Human
- Unigene: 471601 Mouse
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別名
- D2S1471 antibody
- OTTHUMP00000164145 antibody
- VIL antibody
see all
画像
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All lanes : Anti-Villin antibody [EPR3490] (ab109516) at 1/10000 dilution
Lane 1 : Human small intestine lysate
Lane 2 : HT-29 lysate
Lane 3 : Caco-2 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 93 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted? -
Intracellular flow cytometric analysis of Villin in permeabilized HT-29 cells, using ab109515 at a dilution of 1/10 (red) or a rabbit IgG (negative) (green).
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab109516 は 4 報の論文で使用されています。
- Kawai K et al. Establishment of SLC15A1/PEPT1-Knockout Human-Induced Pluripotent Stem Cell Line for Intestinal Drug Absorption Studies. Mol Ther Methods Clin Dev 17:49-57 (2020). PubMed: 31890740
- Benkler C et al. Aggregated SOD1 causes selective death of cultured human motor neurons. Sci Rep 8:16393 (2018). PubMed: 30401824
- Negoro R et al. Modeling of drug-mediated CYP3A4 induction by using human iPS cell-derived enterocyte-like cells. Biochem Biophys Res Commun 472:631-6 (2016). Flow Cyt ; Human . PubMed: 26966071
- Ozawa T et al. Generation of enterocyte-like cells from human induced pluripotent stem cells for drug absorption and metabolism studies in human small intestine. Sci Rep 5:16479 (2015). Flow Cyt ; Human . PubMed: 26559489