Anti-VILIP1 抗体 [EPR9832] (ab180141)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9832] to VILIP1
- Suitable for: Flow Cyt (Intra), IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-VILIP1 antibody [EPR9832]
VILIP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9832] to VILIP1 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IHC-P, WBmore details
適用なし: IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human VILIP1 aa 1-100. The exact sequence is proprietary.
Database link: P62760 -
ポジティブ・コントロール
- Human cerebellum, Mouse brain and rat brain lysate, Human brain tissue, SH-SY5Y cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9832 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab180141の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 22 kDa).
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特記事項 |
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Flow Cyt (Intra)
1/50. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 22 kDa). |
ターゲット情報
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機能
Regulates (in vitro) the inhibition of rhodopsin phosphorylation in a calcium-dependent manner. -
組織特異性
Brain and retina. Neuron-specific in the central and peripheral nervous system. Increased in the cerebrospinal fluid of Alzheimer disease patients (at protein level). -
配列類似性
Belongs to the recoverin family.
Contains 4 EF-hand domains. - Information by UniProt
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参照データベース
- Entrez Gene: 7447 Human
- Entrez Gene: 26950 Mouse
- Entrez Gene: 24877 Rat
- Omim: 600817 Human
- SwissProt: P62760 Human
- SwissProt: P62761 Mouse
- SwissProt: P62762 Rat
- Unigene: 444212 Human
see all -
別名
- 21 kDa CABP antibody
- Hippocalcin like protein 3 antibody
- Hippocalcin-like protein 3 antibody
see all
画像
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All lanes : Anti-VILIP1 antibody [EPR9832] (ab180141) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling VILIP1 with ab180141 at 1/500 dilution. Secondary antibody Goat anti rabbit IgG(HRP) prediluted. Counter stain Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of 2% paraformaldehyde fixedSH-SY5Y cells labeling VILIP1 with ab180141 at 1/50. Secondary antibody Goat anti rabbit IgG (FITC) at 1/75. Isotype control Rabbit IgG.
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Anti-VILIP1 antibody [EPR9832] (ab180141) at 1/1000 dilution + Human cerebellum at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution (Goat Anti-Rabbit IgG HRP)
Predicted band size: 22 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab180141 は論文での使用が確認できていません。