Anti-VGLUT2 抗体 [8G9.2] (ab79157)
Key features and details
- Mouse monoclonal [8G9.2] to VGLUT2
- Suitable for: IHC-Fr, IHC-P, ICC/IF, IHC-FrFl, WB
- Reacts with: Mouse, Rat
- Isotype: IgG1
製品の概要
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製品名
Anti-VGLUT2 antibody [8G9.2]
VGLUT2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [8G9.2] to VGLUT2 -
由来種
Mouse -
特異性
We recommend ab216463 as an alternative for IHC-P and IHC-Fr.
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アプリケーション
適用あり: IHC-Fr, IHC-P, ICC/IF, IHC-FrFl, WBmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Recombinant full length protein corresponding to Rat VGLUT2.
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ポジティブ・コントロール
- Floating mouse brain tissue sections.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: 1.45% Sodium chloride, 0.328% Sodium phosphate -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
8G9.2 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab79157の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
Use at an assay dependent concentration. PubMed: 22064485
We recommend ab216463 as an alternative for IHC-P and IHC-Fr. |
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IHC-P | (3) |
Use at an assay dependent concentration.
We recommend ab216463 as an alternative for IHC-P and IHC-Fr. |
ICC/IF | (1) |
1/1000.
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IHC-FrFl | (1) |
Use at an assay dependent concentration.
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WB | (2) |
Use at an assay dependent concentration. Detects a band of approximately 63 kDa (predicted molecular weight: 64 kDa).
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特記事項 |
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IHC-Fr
Use at an assay dependent concentration. PubMed: 22064485 We recommend ab216463 as an alternative for IHC-P and IHC-Fr. |
IHC-P
Use at an assay dependent concentration. We recommend ab216463 as an alternative for IHC-P and IHC-Fr. |
ICC/IF
1/1000. |
IHC-FrFl
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 63 kDa (predicted molecular weight: 64 kDa). |
ターゲット情報
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機能
Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. -
組織特異性
Predominantly expressed in adult brain. Expressed in amygdala, caudate nucleus, cerebral cortex, frontal lobe, hippocampus, medulla, occipital lobe, putamen, spinal cord, substantia nigra, subthalamic nucleus, temporal lobe and thalamus. -
配列類似性
Belongs to the major facilitator superfamily. Sodium/anion cotransporter family. VGLUT subfamily. -
発生段階
Expressed in fetal brain. -
細胞内局在
Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane. Membrane. Cell junction, synapse, synaptosome. - Information by UniProt
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参照データベース
- Entrez Gene: 140919 Mouse
- Entrez Gene: 84487 Rat
- SwissProt: Q8BLE7 Mouse
- SwissProt: Q9JI12 Rat
- Unigene: 256618 Mouse
- Unigene: 19372 Rat
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別名
- Differentiation associated BNPI antibody
- Differentiation associated Na dependent inorganic phosphate cotransporter antibody
- Differentiation associated Na(+) dependent inorganic phosphate cotransporter antibody
see all
画像
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ab79157, at 1/300 dilution, staining VGLUT2 by Immunohistochemistry using ABC with NiDAB on floating mouse brain tissue sections.
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Immunocytochemistical detection of VGLUT2 using antibody ab79157 on rat primary hippocampal neuronal culture. Fixative: Paraformaldehyde. Permeabilization: Triton 0.3% in 0.1X PBS. Blocking step: 10% Serum as blocking agent for 20 mins @ 24°C. Primary Antibody: 1 µg/ml for 1 hour @ 24°C in Donkey serum in 0.1%PBS plus Triton 0.3%. Secondary Antibody: Alexa Fluor® 568 Conjugation (1/1000). The submitted image shows ab79157 staining rat primary hippocampal neurons.
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All lanes : Anti-VGLUT2 antibody [8G9.2] (ab79157) at 1 µg/ml
Lane 1 : Rat Cortex Tissue Lysate
Lane 2 : Hippocampus (Mouse) Tissue Lysate
Lane 3 : Rat Substantia Nigra Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (40)
ab79157 は 40 報の論文で使用されています。
- Velasco ER et al. PACAP-PAC1R modulates fear extinction via the ventromedial hypothalamus. Nat Commun 13:4374 (2022). PubMed: 35902577
- Mercado NM et al. The BDNF Val66Met polymorphism (rs6265) enhances dopamine neuron graft efficacy and side-effect liability in rs6265 knock-in rats. Neurobiol Dis 148:105175 (2021). PubMed: 33188920
- Humburg BA et al. Optogenetic brain-stimulation reward: A new procedure to re-evaluate the rewarding versus aversive effects of cannabinoids in dopamine transporter-Cre mice. Addict Biol 26:e13005 (2021). PubMed: 33538103
- Florido A et al. Sex differences in fear memory consolidation via Tac2 signaling in mice. Nat Commun 12:2496 (2021). PubMed: 33941789
- Gilissen SRJ et al. Reconsidering the Border between the Visual and Posterior Parietal Cortex of Mice. Cereb Cortex 31:1675-1692 (2021). PubMed: 33159207