リコンビナントRabMAb

Anti-Versican 抗体 [EPR23374-21] (ab270444)

Submit a review Submit a question
Western blot - Anti-Versican antibody [EPR23374-21] (ab270444)
  • Anti-Versican antibody [EPR23374-21] (ab270444)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23374-21] to Versican
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-Versican antibody [EPR23374-21]
    Versican 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23374-21] to Versican

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse brain, Human brain, Rat brain lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Mouse
Human
ICC
Mouse
Human
IHC-Fr
Mouse
Rat
IHC-P
Rat
Human
IP
Mouse
Human
WB
Human
アプリケーション Abreviews 特記事項
WB
1/1000. Detects a band of approximately 370 kDa (predicted molecular weight: 373 kDa).
特記事項
WB
1/1000. Detects a band of approximately 370 kDa (predicted molecular weight: 373 kDa).
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP.

    • ターゲット情報

    • 機能

      May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.

    • 組織特異性

      Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas.

    • 関連疾患

      Defects in VCAN are the cause of Wagner syndrome type 1 (WGN1) [MIM:143200]. WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN.

    • 配列類似性

      Belongs to the aggrecan/versican proteoglycan family.
      Contains 1 C-type lectin domain.
      Contains 2 EGF-like domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
      Contains 2 Link domains.
      Contains 1 Sushi (CCP/SCR) domain.

    • 発生段階

      Disappears after the cartilage development.

    • 翻訳後修飾

      Phosphorylation sites are present in the extracelllular medium.

    • 細胞内局在

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • Chondroitin sulfate proteoglycan 2 antibody
      • Chondroitin sulfate proteoglycan core protein 2 antibody
      • Chondroitin sulfate proteoglycan core protein, cartilage antibody
      • CSPG2 antibody
      • CSPG2_HUMAN antibody
      • ERVR antibody
      • GHAP antibody
      • Glial hyaluronate binding protein antibody
      • Glial hyaluronate-binding protein antibody
      • Large fibroblast proteoglycan antibody
      • PG-M antibody
      • PGM antibody
      • VCAN antibody
      • Versican antibody
      • Versican core protein antibody
      • Versican proteoglycan antibody
      • WGN 1 antibody
      • WGN antibody
      • WGN1 antibody
      see all

    画像

    • Western blot - Anti-Versican antibody [EPR23374-21] (ab270444)
      Western blot - Anti-Versican antibody [EPR23374-21] (ab270444)
      All lanes : Anti-Versican antibody [EPR23374-21] (ab270444) at 1/1000 dilution

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Human brain tissue lysate
      Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate
      Lane 4 : MeWo (human malignant melanoma fibroblast) whole cell lysate
      Lane 5 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

      Predicted band size: 373 kDa
      Observed band size: 370 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer and concentration: 5% NFDM/TBST

      Negative control: MDA-MB-231(PMID:29222454); MeWo(PMID:12972299). 

      Exposure time: 3 minutes

    • Anti-Versican antibody [EPR23374-21] (ab270444)
      Anti-Versican antibody [EPR23374-21] (ab270444)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab270444 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab270444 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab270444.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com