Anti-Versican 抗体 [EPR23374-21] (ab270444)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23374-21] to Versican
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Versican antibody [EPR23374-21]
Versican 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23374-21] to Versican -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Mouse brain, Human brain, Rat brain lysates.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23374-21 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab270444の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000. Detects a band of approximately 370 kDa (predicted molecular weight: 373 kDa).
|
特記事項 |
---|
WB
1/1000. Detects a band of approximately 370 kDa (predicted molecular weight: 373 kDa). |
ターゲット情報
-
機能
May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid. -
組織特異性
Cerebral white matter and plasma. Isoform V0 and isoform V1 are expressed in normal brain, gliomas, medulloblastomas, schwannomas, neurofibromas, and meningiomas. Isoform V2 is restricted to normal brain and gliomas. Isoform V3 is found in all these tissues except medulloblastomas. -
関連疾患
Defects in VCAN are the cause of Wagner syndrome type 1 (WGN1) [MIM:143200]. WGN is a dominantly inherited vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a preretinal avascular membrane. Other optical features include progressive chorioretinal atrophy, perivascular sheating, subcapsular cataract and myopia. Systemic manifestations are absent in WGN. -
配列類似性
Belongs to the aggrecan/versican proteoglycan family.
Contains 1 C-type lectin domain.
Contains 2 EGF-like domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Contains 2 Link domains.
Contains 1 Sushi (CCP/SCR) domain. -
発生段階
Disappears after the cartilage development. -
翻訳後修飾
Phosphorylation sites are present in the extracelllular medium. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
-
参照データベース
- Entrez Gene: 1462 Human
- Entrez Gene: 13003 Mouse
- Entrez Gene: 114122 Rat
- Omim: 118661 Human
- SwissProt: P13611 Human
- SwissProt: Q62059 Mouse
- SwissProt: Q9ERB4 Rat
- Unigene: 643801 Human
see all -
別名
- Chondroitin sulfate proteoglycan 2 antibody
- Chondroitin sulfate proteoglycan core protein 2 antibody
- Chondroitin sulfate proteoglycan core protein, cartilage antibody
see all
画像
-
All lanes : Anti-Versican antibody [EPR23374-21] (ab270444) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Human brain tissue lysate
Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : MeWo (human malignant melanoma fibroblast) whole cell lysate
Lane 5 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 373 kDa
Observed band size: 370 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: MDA-MB-231(PMID:29222454); MeWo(PMID:12972299).
Exposure time: 3 minutes
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (0)
ab270444 は論文での使用が確認できていません。