Application
Immunoprecipitation
An IP was performed using ab175200, the sample was denatured in SDS sample buffer and boiled for 5 min. The sample was then run under denaturing conditions on a 10% gel. AB131366 was used as secondary antibody: 25ul in 5ml 5%-milk-TBST for 30 min at room temperature. No light chain was detected (~25kDa), a bit of heaychain (~50kDa) is still picked up by this antibody.
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投稿 Nov 13 2013