Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lung tissue: Alveolar macrophages)
Antigen retrieval step
None
Permeabilization
No
Specification
Lung tissue: Alveolar macrophages
Blocking step
Zytomed Systems HRP-Polymer Kit Blocking Reagent as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
HOPE-Fixation
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: Zytomed Systems Antibody Diluent
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Zytomed Systems HRP-Polymer Kit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 13 2015