Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (mouse kidneys)
Specification
mouse kidneys
Permeabilization
Yes - 0.3%triton x-100
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 4%BSA, 5% Serum in PBST
Dilution
1/400
Secondary antibody
Name
Non-Abcam antibody was used: DyLight 549 AffiniPure Dnk Anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 546
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 546
Dilution
1/300
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 02 2013