Key features and details
- Rabbit polyclonal to VEGFA
- Suitable for: Sandwich ELISA, WB
- Reacts with: Mouse, Rat, Hamster, Human
- Isotype: IgG
VEGFA 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to VEGFA
特異性Reactive with VEGF 121 as well as VEGF 165.
アプリケーション適用あり: Sandwich ELISA, WBmore details
種交差性交差種: Mouse, Rat, Hamster, Human
交差が予測される動物種: Horse, Guinea pig, Dog, Pig
This product is no longer batch tested in IHC, for an IHC validated antibody please see ab1316.
製品の状態Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Concentration information loading...
精製度Immunogen affinity purified
sELISA pair antibody
Our Abpromise guarantee covers the use of ab9570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGFA (Biotin) (ab83132).
To detect hVEGF by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody as Detection is required. This antigen affinity purified antibody, paired with ab83132, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hVEGF.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.
To detect hVEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
機能Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
組織特異性Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed.
関連疾患Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1 (MVCD1) [MIM:603933]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
配列類似性Belongs to the PDGF/VEGF growth factor family.
細胞内局在Secreted. VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
- Information by UniProt
- Folliculostellate cell-derived growth factor antibody
- Glioma-derived endothelial cell mitogen antibody
- MGC70609 antibody
Lanes 2-12 : Anti-VEGFA antibody (ab9570) at 0.2 µg/ml
Lane 1 : MultiMark MultiColor Standard
Lane 2 : Recombinant Human VEGF at 0.25 µg
Lane 3 : Recombinant Human VEGF at 0.125 µg
Lane 4 : Recombinant Human VEGF at 0.0625 µg
Lane 5 : Recombinant Human VEGF at 0.03125 µg
Lane 6 : Recombinant Human VEGF at 0.015625 µg
Lane 7 : Recombinant Human VEGF at 0.0078 µg
Lane 8 : Recombinant Human VEGF at 0.0039 µg
Lane 9 : Recombinant Human VEGF at 0.00195 µg
Lane 10 : Recombinant Human VEGF at 0.000975 µg
Lane 11 : Recombinant Human VEGF at 0.0004875 µg
Lane 12 : Recombinant Human VEGF at 0.00024 µg
Performed under non-reducing conditions.
Observed band size: 38.2 kDa why is the actual band size different from the predicted?
Detected human VEGF in a sandwich ELISA within the range 16-1000 pg/ml
- 0.5 µg/ml of capture antibody (ab9570)
- 0.25 µg/ml of detection antibody (ab83132)
Lanes 2-12 : Anti-VEGFA antibody (ab9570)
Lane 1 : Molecular weight ladder
Lane 2 : Recombinant human VEGFA at 0.25 µg
Lane 3 : Recombinant human VEGFA at 0.125 µg
Lane 4 : Recombinant human VEGFA at 0.0625 µg
Lane 5 : Recombinant human VEGFA at 0.03125 µg
Lane 6 : Recombinant human VEGFA at 0.015625 µg
Lane 7 : Recombinant human VEGFA at 0.0078 µg
Lane 8 : Recombinant human VEGFA at 0.0039 µg
Lane 9 : Recombinant human VEGFA at 0.00195 µg
Lane 10 : Recombinant human VEGFA at 0.000975 µg
Lane 11 : Recombinant human VEGFA at 0.0004875 µg
Lane 12 : Recombinant human VEGFA at 0.00024 µg
Performed under reducing conditions.
ab9570 は 21 報の論文で使用されています。
- Su X et al. The effect of spleen tyrosine kinase inhibitor R406 on diabetic retinopathy in experimental diabetic rats. Int Ophthalmol N/A:N/A (2020). PubMed: 32462561
- Efeoglu FB et al. Effects of omentin on flap viability: an experimental research on rats. J Plast Surg Hand Surg N/A:1-9 (2019). PubMed: 31244355
- Li Y et al. Artesunate, an Anti-Malaria Agent, Attenuates Experimental Osteoarthritis by Inhibiting Bone Resorption and CD31hiEmcnhi Vessel Formation in Subchondral Bone. Front Pharmacol 10:685 (2019). PubMed: 31258481
- Locri F et al. UPARANT is an effective antiangiogenic agent in a mouse model of rubeosis iridis. J Mol Med (Berl) 97:1273-1283 (2019). PubMed: 31243519
- Yu H et al. Three kinds of corneal host cells contribute differently to corneal neovascularization. EBioMedicine 44:542-553 (2019). PubMed: 31126890