Anti-VEGF Receptor 2 (phospho Y1214) 抗体 (ab5475)

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Western blot - Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475)
  • Western blot - Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475)

Key features and details

  • Rabbit polyclonal to VEGF Receptor 2 (phospho Y1214)
  • Suitable for: WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-VEGF Receptor 2 (phospho Y1214) antibody
    VEGF Receptor 2 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to VEGF Receptor 2 (phospho Y1214)

  • 由来種

    Rabbit

  • 特異性

    VEGFR3 has not been tested, but is expected to react.

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Human, Recombinant fragment
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide corresponding to Human VEGF Receptor 2 (phospho Y1214).

  • ポジティブ･コントロール

    • WB: NIH 3T3 cells transfected with full length, wild-type human VEGFR 2 and Porcine Aortic Endothelial cells transfected with a chimeric receptor consisting of the extracellular domain of the CSF 1 receptor coupled to the transmembrane and cytoplasmic domains of the mouse VEGFR 2. HT-29 Serum Starved followed by treatment for 10 minutes with 100 ng/mL of SCF, Caco-2 Serum Starved followed by treatment for 10 minutes with 100 ng/mL of SCF.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab5475の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use a concentration of 0.35 - 1 µg/ml. Detects a band of approximately 200 kDa.
特記事項
WB
Use a concentration of 0.35 - 1 µg/ml. Detects a band of approximately 200 kDa.

ターゲット情報

  • 機能

    Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.

  • 関連疾患

    Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma.

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.

  • 翻訳後修飾

    Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • CD309 antibody
      • CD309 antigen antibody
      • EC 2.7.10.1 antibody
      • Fetal liver kinase 1 antibody
      • FLK-1 antibody
      • FLK1 antibody
      • FLK1, mouse, homolog of antibody
      • Kdr antibody
      • Kinase insert domain receptor (a type III receptor tyrosine kinase) antibody
      • Kinase insert domain receptor antibody
      • KRD1 antibody
      • Ly73 antibody
      • Protein tyrosine kinase receptor FLK1 antibody
      • Protein-tyrosine kinase receptor flk-1 antibody
      • soluble VEGFR2 antibody
      • Tyrosine kinase growth factor receptor antibody
      • Vascular endothelial growth factor receptor 2 antibody
      • VEGFR 2 antibody
      • VEGFR antibody
      • VEGFR-2 antibody
      • VEGFR2 antibody
      • VGFR2_HUMAN antibody
      see all

    画像

    • Western blot - Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475)
      Western blot - Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475)
      Peptide Competition and Stimulation: Extracts prepared from CSF-1 stimulated (1-4, 6) or unstimulated (5) PAE cells transfected with a chimeric CSF-1/VEGFR2 receptor were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C, then were incubated with 0.50 µg/mL ab5475 antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 5, 6), the nonphosphopeptide corresponding to the immunogen (2), a generic phosphotyrosine containing peptide (3), or, the phosphopeptide immunogen (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5475 blocks the antibody signal, and the stimulation of the phospho signal after stimulating ligand is added (CSF-1 for the chimeric r
    • Western blot - Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475)
      Western blot - Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475)
      All lanes : Anti-VEGF Receptor 2 (phospho Y1214) antibody (ab5475) at 1/1000 dilution

      Lane 1 : HT-29 (Human colorectal adenocarcinoma cell line) whole cell lysate
      Lane 2 : HT-29 Serum Starved followed by treatment for 10 minutes with 100 ng/mL of SCF
      Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate
      Lane 4 : Caco-2 Serum Starved followed by treatment for 10 minutes with 100 ng/mL of SCF

      Lysates/proteins at 30 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/5000 dilution

      Observed band size: ~151 kDa why is the actual band size different from the predicted?



      Modulation of expression of target protein by cell treatment to demonstrate antibody specificity. 

      The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate

    参考文献 (10)

    ab5475 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab5475 は 10 報の論文で使用されています。

    • Hu L  et al. Loss of the RNA-binding protein Rbm15 disrupts liver maturation in zebrafish. J Biol Chem 295:11466-11472 (2020). PubMed: 32518161
    • Chen X  et al. P53-induced miR-1249 inhibits tumor growth, metastasis, and angiogenesis by targeting VEGFA and HMGA2. Cell Death Dis 10:131 (2019). PubMed: 30755600
    • Yang GL  et al. TNFSF15 inhibits VEGF-stimulated vascular hyperpermeability by inducing VEGFR2 dephosphorylation. FASEB J 31:2001-2012 (2017). PubMed: 28183800
    • Arya P  et al. The endocytic recycling regulatory protein EHD1 Is required for ocular lens development. Dev Biol 408:41-55 (2015). PubMed: 26455409
    • Hung YW  et al. Monocyte chemoattractant protein-1 affects migration of hippocampal neural progenitors following status epilepticus in rats. J Neuroinflammation 10:11 (2013). IHC . PubMed: 23339567
    • Liu Z  et al. In vivo Notch reactivation in differentiating cochlear hair cells induces Sox2 and Prox1 expression but does not disrupt hair cell maturation. Dev Dyn 241:684-96 (2012). PubMed: 22354878
    • Färkkilä A  et al. Serum vascular endothelial growth factor A (VEGF) is elevated in patients with ovarian granulosa cell tumor (GCT), and VEGF inhibition by bevacizumab induces apoptosis in GCT in vitro. J Clin Endocrinol Metab 96:E1973-81 (2011). WB, IHC ; Human . PubMed: 21994955
    • Burgess D  et al. Activated Ras alters lens and corneal development through induction of distinct downstream targets. BMC Dev Biol 10:13 (2010). IHC . PubMed: 20105280
    • Yeh J  et al. Ovarian vascular endothelial growth factor and vascular endothelial growth factor receptor patterns in reproductive aging. Fertil Steril 89:1546-56 (2008). PubMed: 17889862
    • Wu L  et al. A simple method for obtaining transferrins from human plasma and porcine serum: preparations and properties. J Chromatogr B Analyt Technol Biomed Life Sci 867:62-8 (2008). PubMed: 18396113

    レビューと Q&A

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    Question

    I have a question about Flk-1 antibody. Does Flk-1 expressed in all endothelial cells or just active endothelial cells, like with agiogenesis. I did a stain for mouse heart by use, CD31 ( pharmingen), Von factor VIII( abcam), and Flk-1, Both CD31 and VIII are positive in heart vessels, but Flk-1 is negative, I don't know if it is because of antibody problem, what if I use ab5475? Thanks, Sincerely,

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    Answer

    Thank you for your enquiry. This antibody will recognize VEGFR-2 only if it is phosphorylated on Y1214.We would like to emphasize the fact that this antibody has only been tested for Western blot application.

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