Anti-VEGF Receptor 2 抗体 [EPR21884-290] (ab221679)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21884-290] to VEGF Receptor 2
- Suitable for: WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
製品の概要
-
製品名
Anti-VEGF Receptor 2 antibody [EPR21884-290]
VEGF Receptor 2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR21884-290] to VEGF Receptor 2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Mouse and rat placenta and lung lysates; bEnd.3 whole cell lysate.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR21884-290 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab221679の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000. Detects a band of approximately 180-230 kDa (predicted molecular weight: 152 kDa).
|
特記事項 |
---|
WB
1/1000. Detects a band of approximately 180-230 kDa (predicted molecular weight: 152 kDa). |
ターゲット情報
-
機能
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
関連疾患
Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 16542 Mouse
- Entrez Gene: 25589 Rat
- SwissProt: P35918 Mouse
- SwissProt: O08775 Rat
- Unigene: 285 Mouse
- Unigene: 88869 Rat
-
別名
- CD309 antibody
- CD309 antigen antibody
- EC 2.7.10.1 antibody
see all
画像
-
All lanes : Anti-VEGF Receptor 2 antibody [EPR21884-290] (ab221679) at 1/1000 dilution
Lane 1 : Mouse placenta lysate at 20 µg
Lane 2 : Rat placenta lysate at 20 µg
Lane 3 : Rat lung lysate at 20 µg
Lane 4 : Mouse lung lysate at 20 µg
Lane 5 : bEnd.3 (mouse brain endothelioma cell line) whole cell lysate at 10 µg
Secondary
Lanes 1-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 152 kDa
Observed band size: 180-230 kDa why is the actual band size different from the predicted?Exposure time : Lanes 1-2: 3 minutes; Lane 3: 37 seconds; Lane 4: 59 seconds; Lane 5: 15 seconds
The molecular mass observed is consistent with the literature (PMID: 23354168 ; PMID: 20606037).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (4)
ab221679 は 4 報の論文で使用されています。
- Jiang X et al. Overexpression of Pregnancy Zone Protein in Fat Antagonizes Diet-Induced Obesity Under an Intermittent Fasting Regime. Front Physiol 13:950619 (2022). PubMed: 36051914
- Song J et al. Apatinib suppresses the migration, invasion and angiogenesis of hepatocellular carcinoma cells by blocking VEGF and PI3K/AKT signaling pathways. Mol Med Rep 23:N/A (2021). PubMed: 33846786
- Wang Y & Jiang L Role of vitamin D-vitamin D receptor signaling on hyperoxia-induced bronchopulmonary dysplasia in neonatal rats. Pediatr Pulmonol 56:2335-2344 (2021). PubMed: 33878208
- Lin W et al. RUNX1/EGFR pathway contributes to STAT3 activation and tumor growth caused by hyperactivated mTORC1. Mol Ther Oncolytics 23:387-401 (2021). PubMed: 34853810