Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (iCell® Cardiomyocytes)
Permeabilization
No
Specification
iCell® Cardiomyocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Acetone
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% FBS
Dilution
1/400
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa Fluor 488 Goat Anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
This is the best image I have which should good co-staining with nuclear marker DAPI.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 25 2015