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  1. Link

    vegf-receptor-1-antibody-ab2350.pdf

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Cardiovascular Angiogenesis Growth Factors VEGF VEGF Receptors
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Anti-VEGF Receptor 1 抗体 (ab2350)

  • Datasheet
  • SDS
Reviews (2)Q&A (5)References (58)

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Abpromise

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Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 1 antibody (ab2350)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody (ab2350)

Key features and details

  • Rabbit polyclonal to VEGF Receptor 1
  • Suitable for: WB, IHC-P, ICC/IF, Electron Microscopy
  • Reacts with: Mouse, Rat, Human, Pig
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-VEGF Receptor 1 antibody [Y103] - Low endotoxin, Azide free (ab184784)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-VEGF Receptor 1 antibody
    VEGF Receptor 1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to VEGF Receptor 1
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, IHC-P, ICC/IF, Electron Microscopymore details
  • 種交差性

    交差種: Mouse, Rat, Human, Pig
  • 免疫原

    Synthetic peptide within Human VEGF Receptor 1 aa 1300 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P17948

  • ポジティブ・コントロール

    • IHC-P: Human angiosarcoma tissue. ICC/IF: A431 cell line
  • 特記事項

    VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 一次抗体 備考

    VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • VEGF
    • VEGF Receptors
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Growth Factors/Hormones
    • VEGF
    • Cancer
    • Growth factors
    • VEGF
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factors
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers
    • Neuroscience
    • Development
    • Neuroscience
    • Processes

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human VEGF Receptor 1 protein (ab54346)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2350の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 151 kDa. PubMed: 21912702
IHC-P (2)
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 5 µg/ml.
Electron Microscopy
Use at an assay dependent concentration. PubMed: 18647955
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 151 kDa. PubMed: 21912702
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
Use a concentration of 5 µg/ml.
Electron Microscopy
Use at an assay dependent concentration. PubMed: 18647955

ターゲット情報

  • 機能

    Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
  • 組織特異性

    Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta.
  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 細胞内局在

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P17948 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 2321 Human
    • Entrez Gene: 14254 Mouse
    • Entrez Gene: 54251 Rat
    • Omim: 165070 Human
    • SwissProt: P17948 Human
    • SwissProt: P35969 Mouse
    • SwissProt: P53767 Rat
    • Unigene: 594454 Human
    • Unigene: 389712 Mouse
    • Unigene: 10239 Rat
    see all
  • 別名

    • EC 2.7.10.1 antibody
    • FLT 1 antibody
    • FLT antibody
    • Flt-1 antibody
    • FLT1 antibody
    • Fms like tyrosine kinase 1 antibody
    • Fms related tyrosine kinase 1 antibody
    • Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) antibody
    • Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor antibody
    • Fms-like tyrosine kinase 1 antibody
    • FRT antibody
    • Soluble VEGF receptor 1 14 antibody
    • Soluble VEGFR1 variant 2 antibody
    • Soluble VEGFR1 variant 21 antibody
    • Tyrosine protein kinase FRT antibody
    • Tyrosine protein kinase receptor FLT antibody
    • Tyrosine-protein kinase FRT antibody
    • Tyrosine-protein kinase receptor FLT antibody
    • Vascular endothelial growth factor receptor 1 antibody
    • Vascular endothelial growth factor vascular permeability factor receptor antibody
    • Vascular permeability factor receptor 1 antibody
    • Vascular permeability factor receptor antibody
    • VEGFR 1 antibody
    • VEGFR-1 antibody
    • VEGFR1 antibody
    • VGFR1_HUMAN antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 1 antibody (ab2350)
    Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 1 antibody (ab2350)

    ICC/IF image of ab2350 stained A431 (human epidermoid carcinoma cell line) cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2350, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody (ab2350)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody (ab2350)

    ab2350 - immunohistochemistry

    Formalin fixed paraffin embedded human angiosarcoma stained with VEGF Receptor 1, using ABC and AEC chromogen.

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (58)

ab2350 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2350 は 58 報の論文で使用されています。

  • Lu C  et al. Tumor necrosis factor receptor type 1-associated death domain (TRADD) regulates epithelial-mesenchymal transition (EMT), M1/M2 macrophage polarization and ectopic endometrial cysts formation in endometriosis. Ann Transl Med 9:148 (2021). PubMed: 33569450
  • Qu H  et al. HIF-3a affects preeclampsia development by regulating EVT growth via activation of the Flt-1/JAK/STAT signaling pathway in hypoxia. Mol Med Rep 23:N/A (2021). PubMed: 33215219
  • Li J  et al. A pentapeptide enabled AL3810 liposome-based glioma-targeted therapy with immune opsonic effect attenuated. Acta Pharm Sin B 11:283-299 (2021). PubMed: 33532193
  • Kim SH  et al. Porcine endometrial 3D co-culture: Morphological changes in 3D endometrium tissues according to hormonal changes. Histol Histopathol N/A:18335 (2021). PubMed: 33829420
  • Silva JAF  et al. Spatial and temporal VEGF receptor intracellular trafficking in microvascular and macrovascular endothelial cells. Sci Rep 11:17400 (2021). PubMed: 34462507
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-7 of 7 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VEGF Receptor 1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 12 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VEGF Receptor 1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Specification
lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Manoj Kumar Valluru

Verified customer

投稿 Jun 11 2010

Question

Could you please tell me if this antibody has already been tested on canine tissue?

Read More

Abcam community

Verified customer

Asked on Feb 22 2007

Answer

Thank you for your enquiry. This antibody has not been tested on canine tissues. We do not give discounts on products in order to test the antibody, but we do have a rewards program. If you use the antibody on canine tissue, please consider our AbTrial testing program. For more information see www.abcam.com/abtrial

Read More

Abcam Scientific Support

Answered on Feb 22 2007

Question

What do you mean by amplification?

Read More

Abcam community

Verified customer

Asked on Mar 07 2005

Answer

Thank you for your e-mail. Amplification is a method to amplify signal on immunohistochemical preparations where there is low abundance of the protein of interest (or the primary antibody binding affinity for the protein is low). The most common amplification method is the ABC method, where this procedure employs biotinylated antibody and a preformed Avidin: Biotinylated enzyme Complex and has been termed the “ABC” technique. Because avidin has such an extraordinarily high affinity for biotin (over one million times higher than antibody for most antigens), the binding of avidin to biotin is essentially irreversible. In addition, avidin has four binding sites for biotin, and most proteins including enzymes can be conjugated with several molecules of biotin. The high sensitivity reported for this technique likely resides in either the form or the number of active enzyme molecules associated with the complex and the rapid, irreversible interaction of the complex with biotinylated antibody. In addition to high sensitivity, ABC kits produce very low background staining. In summary, following incubation of the primary antibody (rabbit), wash in PBS your sections/cells, then add an anti-rabbit secondary antibody which is biotin conjugated, incubate for 2-3 hours, then wash in PBS and add HRP-conjugated avidin (provided in a kit, such as the Vector ABC kit). The avidin will bind to the biotin with high affinity and provide an amplification. When DAB will be added at the last step the numerous HRP proteins will give more signal and you will be able to visualise the localisation of your protein of interest.

Read More

Abcam Scientific Support

Answered on Mar 08 2005

Question

I have just purchased your product ab2350 (VEGF Receptor 1 antibody)and there is no indication about dilution for western blot in its data sheet. Which starting dilution do you suggest?

Read More

Abcam community

Verified customer

Asked on Sep 28 2004

Answer

I would recommend a preliminary experiment using a range of dilutions: 1:200, 1:500, 1:1000, 1:3000 for example and optimise the dilution following the outcome of this experiment.

Read More

Abcam Scientific Support

Answered on Sep 28 2004

Question

We received an inquiry from one of our customer about your product of #AB2350. Could you please let us know whether #AB2350 recognize Phospho-VEGF Receptor 1,or not ? We look forward to receiving your early reply.

Read More

Abcam community

Verified customer

Asked on Oct 13 2003

Answer

We do not have any data on the whether it recognises the phospho VEGFR1. We would guess that it would detect both phosphorylated and non-phosphorylated since it was raised against a non-phosphorylated peptide. However, we would be very interested to hear if this is the case

Read More

Asdf Edo

Abcam Scientific Support

Answered on Oct 13 2003

Question

What is the peptide sequence against which this antibody is raised? I would be interested to do a blast search for that sequence to see if it shows any degree of homolgy to sheep VEGF receptor.

Read More

Abcam community

Verified customer

Asked on Jul 24 2003

Answer

The synthetic peptide sequence for Flt-1 Ab-1: a.a. 1312-1328 (Cys-AELERKIACCSPPPDYN)

Read More

Abcam Scientific Support

Answered on Sep 09 2003

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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