製品の概要

  • 製品名
    Anti-VEGF Receptor 1 antibody
    VEGF Receptor 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to VEGF Receptor 1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: IHC-P, Electron Microscopy, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Pig
  • 免疫原

    Synthetic peptide within Human VEGF Receptor 1 aa 1300 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P17948

  • ポジティブ･コントロール
    • IHC-P: Mouse lung tissue; human angiosarcoma tissue. ICC/IF: A431 cell line
  • 特記事項


    VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Electron Microscopy 1/100. PubMed: 18647955
WB Use at an assay dependent concentration. PubMed: 21912702
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
  • 組織特異性
    Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta.
  • 配列類似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 細胞内局在
    Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • EC 2.7.10.1 antibody
    • FLT 1 antibody
    • FLT antibody
    • Flt-1 antibody
    • FLT1 antibody
    • Fms like tyrosine kinase 1 antibody
    • Fms related tyrosine kinase 1 antibody
    • Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) antibody
    • Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor antibody
    • Fms-like tyrosine kinase 1 antibody
    • FRT antibody
    • Soluble VEGF receptor 1 14 antibody
    • Soluble VEGFR1 variant 2 antibody
    • Soluble VEGFR1 variant 21 antibody
    • Tyrosine protein kinase FRT antibody
    • Tyrosine protein kinase receptor FLT antibody
    • Tyrosine-protein kinase FRT antibody
    • Tyrosine-protein kinase receptor FLT antibody
    • Vascular endothelial growth factor receptor 1 antibody
    • Vascular endothelial growth factor vascular permeability factor receptor antibody
    • Vascular permeability factor receptor 1 antibody
    • Vascular permeability factor receptor antibody
    • VEGFR 1 antibody
    • VEGFR-1 antibody
    • VEGFR1 antibody
    • VGFR1_HUMAN antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody (ab2350)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody (ab2350)This image is courtesy of an Abreview submitted by Manoj Kumar Valluru
    ab2350 staining the VEGF Receptor 1 in Mouse lung tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in citrate buffer (pH 6). Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An undiluted biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 1 antibody (ab2350)
    Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 1 antibody (ab2350)

    ICC/IF image of ab2350 stained A431 (human epidermoid carcinoma cell line) cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2350, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody (ab2350)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody (ab2350)

    ab2350 - immunohistochemistry

    Formalin fixed paraffin embedded human angiosarcoma stained with VEGF Receptor 1, using ABC and AEC chromogen.

参考文献

This product has been referenced in:

See all 32 Publications for this product

ab2350 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

7 reviews or Q&A

10
Votes: Highest First

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VEGF Receptor 1 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
Lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
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Username

Abcam user community

Verified customer

投稿 May 12 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VEGF Receptor 1 antibody

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Specification
lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Read More
abreview image
Username

Mr. Manoj Kumar Valluru

Verified customer

投稿 Jun 11 2010

Could you please tell me if this antibody has already been tested on canine tissue?

Thank you for your enquiry. This antibody has not been tested on canine tissues. We do not give discounts on products in order to test the antibody, but we do have a rewards program. If you use the antibody on canine tissue, please consider our AbTrial...

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What do you mean by amplification?

Thank you for your e-mail. Amplification is a method to amplify signal on immunohistochemical preparations where there is low abundance of the protein of interest (or the primary antibody binding affinity for the protein is low). The most common ...

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I have just purchased your product ab2350 (VEGF Receptor 1 antibody)and there is no indication about dilution for western blot in its data sheet. Which starting dilution do you suggest?

I would recommend a preliminary experiment using a range of dilutions: 1:200, 1:500, 1:1000, 1:3000 for example and optimise the dilution following the outcome of this experiment.

We received an inquiry from one of our customer about your product of #AB2350. Could you please let us know whether #AB2350 recognize Phospho-VEGF Receptor 1,or not ? We look forward to receiving your early reply.

We do not have any data on the whether it recognises the phospho VEGFR1. We would guess that it would detect both phosphorylated and non-phosphorylated since it was raised against a non-phosphorylated peptide. However, we would be very interested to he...

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What is the peptide sequence against which this antibody is raised? I would be interested to do a blast search for that sequence to see if it shows any degree of homolgy to sheep VEGF receptor.

The synthetic peptide sequence for Flt-1 Ab-1: a.a. 1312-1328 (Cys-AELERKIACCSPPPDYN)

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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