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  1. Link

    vegf-receptor-1-antibody-ab2350.pdf

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Cardiovascular Angiogenesis Growth Factors VEGF VEGF Receptors
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Anti-VEGF Receptor 1 抗体 (ab2350)

  • Datasheet
  • SDS
Reviews (2)Q&A (5)References (49)

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 1 antibody (ab2350)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody (ab2350)

Key features and details

  • Rabbit polyclonal to VEGF Receptor 1
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
タンパク質
Product image
Recombinant human VEGF Receptor 1 protein (ab54346)

関連製品

製品の概要

  • 製品名

    Anti-VEGF Receptor 1 antibody
    VEGF Receptor 1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to VEGF Receptor 1
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-P, ICC/IFmore details
  • 種交差性

    交差種: Human
    交差が予測される動物種: Pig
  • 免疫原

    Synthetic peptide within Human VEGF Receptor 1 aa 1300 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P17948

  • ポジティブ・コントロール

    • IHC-P: Human angiosarcoma tissue. ICC/IF: A431 cell line
  • 特記事項

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.


    VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 一次抗体 備考

    VEGFR 1 is a cell membrane receptor kinase. It has a high affinity receptor for vascular endothelial growth factor and is putatively involved in the growth of endothelial cells and angiogenesis.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cardiovascular
    • Angiogenesis
    • Growth Factors
    • VEGF
    • VEGF Receptors
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Signal Transduction
    • Growth Factors/Hormones
    • VEGF
    • Cancer
    • Growth factors
    • VEGF
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factors
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human VEGF Receptor 1 protein (ab54346)

アプリケーション

Our Abpromise guarantee covers the use of ab2350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能

    Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis.
  • 組織特異性

    Mostly in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform sFlt1 is strongly expressed in placenta.
  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 細胞内局在

    Secreted and Cell membrane.
  • Target information above from: UniProt accession P17948 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 2321 Human
    • Omim: 165070 Human
    • SwissProt: P17948 Human
    • Unigene: 594454 Human
    • 別名

      • EC 2.7.10.1 antibody
      • FLT 1 antibody
      • FLT antibody
      • Flt-1 antibody
      • FLT1 antibody
      • Fms like tyrosine kinase 1 antibody
      • Fms related tyrosine kinase 1 antibody
      • Fms related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) antibody
      • Fms related tyrosine kinase 1 vascular endothelial growth factor/vascular permeability factor receptor antibody
      • Fms-like tyrosine kinase 1 antibody
      • FRT antibody
      • Soluble VEGF receptor 1 14 antibody
      • Soluble VEGFR1 variant 2 antibody
      • Soluble VEGFR1 variant 21 antibody
      • Tyrosine protein kinase FRT antibody
      • Tyrosine protein kinase receptor FLT antibody
      • Tyrosine-protein kinase FRT antibody
      • Tyrosine-protein kinase receptor FLT antibody
      • Vascular endothelial growth factor receptor 1 antibody
      • Vascular endothelial growth factor vascular permeability factor receptor antibody
      • Vascular permeability factor receptor 1 antibody
      • Vascular permeability factor receptor antibody
      • VEGFR 1 antibody
      • VEGFR-1 antibody
      • VEGFR1 antibody
      • VGFR1_HUMAN antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 1 antibody (ab2350)
      Immunocytochemistry/ Immunofluorescence - Anti-VEGF Receptor 1 antibody (ab2350)

      ICC/IF image of ab2350 stained A431 (human epidermoid carcinoma cell line) cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2350, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody (ab2350)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VEGF Receptor 1 antibody (ab2350)

      ab2350 - immunohistochemistry

      Formalin fixed paraffin embedded human angiosarcoma stained with VEGF Receptor 1, using ABC and AEC chromogen.

    プロトコール

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (49)

    ab2350 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2350 は 49 報の論文で使用されています。

    • Lin Z  et al. The effect of overexpression of the HOXD10 gene on the malignant proliferation, invasion, and tumor formation of pancreatic cancer cell PANC-1. Gland Surg 9:385-391 (2020). PubMed: 32420263
    • Kurowska P  et al. Novel Insights on the Corpus Luteum Function: Role of Vaspin on Porcine Luteal Cell Angiogenesis, Proliferation and Apoptosis by Activation of GRP78 Receptor and MAP3/1 Kinase Pathways. Int J Mol Sci 21:N/A (2020). PubMed: 32957618
    • Liao W  et al. BMSC-derived exosomes carrying microRNA-122-5p promote proliferation of osteoblasts in osteonecrosis of the femoral head. Clin Sci (Lond) 133:1955-1975 (2019). PubMed: 31387936
    • Stassi AF  et al. Contribution of the VEGF system to the follicular persistence associated with bovine cystic ovaries. Theriogenology 138:52-65 (2019). PubMed: 31301448
    • Su J  et al. Ultrasound-mediated destruction of vascular endothelial growth factor (VEGF) targeted and paclitaxel loaded microbubbles for inhibition of human breast cancer cell MCF-7 proliferation. Mol Cell Probes 46:101415 (2019). PubMed: 31228519
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VEGF Receptor 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    No
    Specification
    Lung
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 May 12 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-VEGF Receptor 1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (lung)
    Specification
    lung
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Read More

    Mr. Manoj Kumar Valluru

    Verified customer

    投稿 Jun 11 2010

    Question

    Could you please tell me if this antibody has already been tested on canine tissue?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 22 2007

    Answer

    Thank you for your enquiry. This antibody has not been tested on canine tissues. We do not give discounts on products in order to test the antibody, but we do have a rewards program. If you use the antibody on canine tissue, please consider our AbTrial testing program. For more information see www.abcam.com/abtrial

    Read More

    Abcam Scientific Support

    Answered on Feb 22 2007

    Question

    What do you mean by amplification?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 07 2005

    Answer

    Thank you for your e-mail. Amplification is a method to amplify signal on immunohistochemical preparations where there is low abundance of the protein of interest (or the primary antibody binding affinity for the protein is low). The most common amplification method is the ABC method, where this procedure employs biotinylated antibody and a preformed Avidin: Biotinylated enzyme Complex and has been termed the “ABC” technique. Because avidin has such an extraordinarily high affinity for biotin (over one million times higher than antibody for most antigens), the binding of avidin to biotin is essentially irreversible. In addition, avidin has four binding sites for biotin, and most proteins including enzymes can be conjugated with several molecules of biotin. The high sensitivity reported for this technique likely resides in either the form or the number of active enzyme molecules associated with the complex and the rapid, irreversible interaction of the complex with biotinylated antibody. In addition to high sensitivity, ABC kits produce very low background staining. In summary, following incubation of the primary antibody (rabbit), wash in PBS your sections/cells, then add an anti-rabbit secondary antibody which is biotin conjugated, incubate for 2-3 hours, then wash in PBS and add HRP-conjugated avidin (provided in a kit, such as the Vector ABC kit). The avidin will bind to the biotin with high affinity and provide an amplification. When DAB will be added at the last step the numerous HRP proteins will give more signal and you will be able to visualise the localisation of your protein of interest.

    Read More

    Abcam Scientific Support

    Answered on Mar 08 2005

    Question

    I have just purchased your product ab2350 (VEGF Receptor 1 antibody)and there is no indication about dilution for western blot in its data sheet. Which starting dilution do you suggest?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 28 2004

    Answer

    I would recommend a preliminary experiment using a range of dilutions: 1:200, 1:500, 1:1000, 1:3000 for example and optimise the dilution following the outcome of this experiment.

    Read More

    Abcam Scientific Support

    Answered on Sep 28 2004

    Question

    We received an inquiry from one of our customer about your product of #AB2350. Could you please let us know whether #AB2350 recognize Phospho-VEGF Receptor 1,or not ? We look forward to receiving your early reply.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 13 2003

    Answer

    We do not have any data on the whether it recognises the phospho VEGFR1. We would guess that it would detect both phosphorylated and non-phosphorylated since it was raised against a non-phosphorylated peptide. However, we would be very interested to hear if this is the case

    Read More

    Asdf Edo

    Abcam Scientific Support

    Answered on Oct 13 2003

    Question

    What is the peptide sequence against which this antibody is raised? I would be interested to do a blast search for that sequence to see if it shows any degree of homolgy to sheep VEGF receptor.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 24 2003

    Answer

    The synthetic peptide sequence for Flt-1 Ab-1: a.a. 1312-1328 (Cys-AELERKIACCSPPPDYN)

    Read More

    Abcam Scientific Support

    Answered on Sep 09 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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