Anti-VE Cadherin (phospho Y685) 抗体 (ab119785)
Key features and details
- Rabbit polyclonal to VE Cadherin (phospho Y685)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-VE Cadherin (phospho Y685) antibody
VE Cadherin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to VE Cadherin (phospho Y685) -
由来種
Rabbit -
特異性
ab119785 detects a 140 kDa band corresponding to VE cadherin in western blots of Human endothelial cells treated with pervanadate, and this band is not detected in untreated cells or after alkaline phosphatase treatment. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human VE Cadherin (phospho Y685).
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ポジティブ・コントロール
- Human umbilical vein endothelial cells stimulated with pervanadate.
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特記事項
Do not aliquot.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab119785 was cross-adsorbed to an unrelated phospho-tyrosine peptide and unphosphorylated VE Cadherin (Y685) peptide before affinity purification using phospho-VE Cadherin (Y685) peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab119785の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 88 kDa).
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特記事項 |
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WB
1/1000. Detects a band of approximately 140 kDa (predicted molecular weight: 88 kDa). |
ターゲット情報
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機能
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. -
組織特異性
Endothelial tissues and brain. -
配列類似性
Contains 5 cadherin domains. -
翻訳後修飾
Phosphorylated on tyrosine residues by KDR/VEGFR-2. Dephosphorylated by PTPRB. -
細胞内局在
Cell junction. Cell membrane. Found at cell-cell boundaries and probably at cell-matrix boundaries. - Information by UniProt
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参照データベース
- Entrez Gene: 1003 Human
- Entrez Gene: 12562 Mouse
- Entrez Gene: 307618 Rat
- Omim: 601120 Human
- SwissProt: P33151 Human
- SwissProt: P55284 Mouse
- Unigene: 76206 Human
- Unigene: 21767 Mouse
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別名
- 7B 4 antibody
- 7B4 antibody
- 7B4 antigen antibody
see all
画像
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All lanes : Anti-VE Cadherin (phospho Y685) antibody (ab119785) at 1/1000 dilution
Lane 1 : Human umbilical vein endothelial
cells stimulated with pervanadate (1 mM) for 30 min.
Lane 2 : Human umbilical vein endothelial
cells stimulated with pervanadate (1 mM) for 30 min then treated with
alkaline phosphatase
Predicted band size: 88 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (26)
ab119785 は 26 報の論文で使用されています。
- Kubra KT et al. Tunicamycin Protects against LPS-Induced Lung Injury. Pharmaceuticals (Basel) 15:N/A (2022). PubMed: 35215247
- Nyandwi JB et al. Rosmarinic acid downregulates the oxLDL‑induced interaction between monocytes and endothelial cells, in addition to monocyte diapedesis, under high glucose conditions. Int J Mol Med 49:N/A (2022). PubMed: 35315501
- Liu MM et al. Diammonium glycyrrhizinate lipid ligand ameliorates lipopolysaccharide-induced acute lung injury by modulating vascular endothelial barrier function. Exp Ther Med 21:303 (2021). PubMed: 33717246
- Baganha F et al. Atorvastatin pleiotropically decreases intraplaque angiogenesis and intraplaque haemorrhage by inhibiting ANGPT2 release and VE-Cadherin internalization. Angiogenesis 24:567-581 (2021). PubMed: 33550461
- Su K et al. YAP expression in endothelial cells prevents ventilator-induced lung injury. Am J Physiol Lung Cell Mol Physiol 320:L568-L582 (2021). PubMed: 33565367