Application
Immunocytochemistry
Sample
Pig Cultured Cells (Endothelial cell)
Permeabilization
Yes - Methanol
Specification
Endothelial cell
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Methanol
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Goat anti-Rabbit Cy3 IgG-HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
All the staining were on cell nuclear not on cell junction and membrane as data sheet described. Green is the cytoplasm marker, blue is DAPI, red is VE Cadherin (ab33168).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 10 2018