Anti-VDAC1/Porin 抗体 - Mitochondrial Loading Control (ab15895)
Key features and details
- Rabbit polyclonal to VDAC1/Porin - Mitochondrial Loading Control
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Chicken, Dog, Human, Chinese hamster
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-VDAC1/Porin antibody - Mitochondrial Loading Control
VDAC1/Porin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to VDAC1/Porin - Mitochondrial Loading Control -
由来種
Rabbit -
特異性
This antibody detects VDAC1, VDAC2 and VDAC3. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Chicken, Dog, Human, Chinese hamster
交差が予測される動物種: Rabbit, Cow, Pig, Zebrafish
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- This antibody gave a positive control in the following human whole cell lysates: HeLa A431 Jurkat HEK293 This antibody gave a positive control in HeLa (Human epithelial carcinoma cell line) Nuclear This antibody gave a positive control in the following mouse tissue lysates: Heart Kidney Skeletal Muscle Spinal Cord This antibody gave a positive control in the following rat lysates: PC12 Whole Cell Brain Tissue Lysate - normal tissue Kidney Whole Cell Lysate - normal tissue
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15895の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (20) |
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
Abcam recommends using BSA as the blocking agent. |
| ICC/IF | (1) |
Use a concentration of 5 µg/ml.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa). Abcam recommends using BSA as the blocking agent. |
|
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis. -
組織特異性
Heart, liver and skeletal muscle. -
配列類似性
Belongs to the eukaryotic mitochondrial porin family. -
ドメイン
Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity. -
細胞内局在
Mitochondrion outer membrane. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 416320 Chicken
- Entrez Gene: 282119 Cow
- Entrez Gene: 7416 Human
- Entrez Gene: 22333 Mouse
- Entrez Gene: 397010 Pig
- Entrez Gene: 100008751 Rabbit
- Entrez Gene: 83529 Rat
- Entrez Gene: 334582 Zebrafish
see all -
別名
- N2441 antibody
- OMP2 antibody
- POR1 antibody
see all
画像
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Western blot - Anti-VDAC1/Porin antibody - Mitochondrial Loading Control (ab15895)All lanes : Anti-VDAC1/Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Hela Nuclear lysate
Lane 2 : Hela cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : Hela Nuclear lysate with Human VDAC1/Porin peptide (ab16131) at 1 µg/ml
Lane 7 : Hela cell lysate with Human VDAC1/Porin peptide (ab16131) at 1 µg/ml
Lane 8 : A431 cell lysate with Human VDAC1/Porin peptide (ab16131) at 1 µg/ml
Lane 9 : Jurkat cell lysate with Human VDAC1/Porin peptide (ab16131) at 1 µg/ml
Lane 10 : HEK293 cell lysate with Human VDAC1/Porin peptide (ab16131) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 31 kDa -
Western blot - Anti-VDAC1/Porin antibody - Mitochondrial Loading Control (ab15895)All lanes : Anti-VDAC1/Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 :Mouse skeletal muscle tissue lysate - total protein (ab29711)
Lane 4 : Spinal Cord (Mouse) Tissue Lysate
Lane 5 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 6 : Brain (Rat) Tissue Lysate - normal tissue
Lane 7 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 31 kDa -
Immunocytochemistry - Anti-VDAC1/Porin antibody - Mitochondrial Loading Control (ab15895)ab15895 staining VDAC1 / Porin in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab15895 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Western blot - Anti-VDAC1/Porin antibody - Mitochondrial Loading Control (ab15895)All lanes : Anti-VDAC1/Porin antibody - Mitochondrial Loading Control (ab15895) at 1 µg/ml
Lane 1 : Chicken liver cell lysate
Lane 2 : CHO K1 cell lysate
Lane 3 : MDCK cell lysate
Lane 4 : Chicken liver cell lysate with Human VDAC1/Porin peptide (ab16131) at 1 µg/ml
Lane 5 : CHO K1 cell lysate with Human VDAC1/Porin peptide (ab16131) at 1 µg/ml
Lane 6 : MDCK cell lysate with Human VDAC1/Porin peptide (ab16131) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa fluor goat polyclonal anti-Rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 31 kDa -
Western blot - Anti-VDAC1/Porin antibody - Mitochondrial Loading Control (ab15895)Western blot image using the Optiblot Reducing Electrophoresis Kit - 10 x 10 cm (4-20%) (ab119220) with the
Prism Ultra Protein Ladder (ab116028) 5µl used. We recommend using our ECL substrate kit (ab65623) .
20ug of Lysate per lane and detection using ab15895 diluted to 1ug/ml.
Lane 1: HeLa cell lysate
Lane 2: Jurkat cell lysate
Lane 3: A431 cell lysate
Lane 4: HEK293 cell lysate
Lane 5: HepG2 cell lysate.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (322)
ab15895 は 322 報の論文で使用されています。
- Franco-Iborra S et al. Mutant HTT (huntingtin) impairs mitophagy in a cellular model of Huntington disease. Autophagy 17:672-689 (2021). PubMed: 32093570
- Mehta AR et al. Mitochondrial bioenergetic deficits in C9orf72 amyotrophic lateral sclerosis motor neurons cause dysfunctional axonal homeostasis. Acta Neuropathol 141:257-279 (2021). PubMed: 33398403
- Wersäll OC et al. PGC1a and VDAC1 expression in endometrial cancer. Mol Clin Oncol 14:42 (2021). PubMed: 33437480
- Sotome R et al. In vivo emergence of beige-like fat in chickens as physiological adaptation to cold environments. Amino Acids 53:381-393 (2021). PubMed: 33598768
- Lee J et al. Loss of primary cilia promotes mitochondria-dependent apoptosis in thyroid cancer. Sci Rep 11:4181 (2021). PubMed: 33602982