Application
Western blot
Sample
Rat Cell lysate - other (brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: skim milk in TBS-T
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: Mouse IgG (NA931V)
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
Brain mitochondrial lysate
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 39 kDa
Positive control
Brain homogenate
Additional data
Additional Notes
For image -
H = brain homogenate
M = brain mitochondrial lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Jun 19 2007