Application
Western blot
Sample
Human Cell lysate - whole cell (Human primary fibroblasts)
Gel Running Conditions
Reduced Denaturing (12% TGX)
Loading amount
30 µg
Specification
Human primary fibroblasts
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST (0.1% Tween), 3% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Donkey anti-mouse HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
1 second(s)
Bands
Specific: 31 kDa Non-specific: 39, 41, 45, 49 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 02 2021