Application
Western blot
Sample
Human Cell lysate - other (U2OS Cells Crude Fractionation (Cytosol v Membrane)
Gel Running Conditions
Reduced Denaturing (4-20% Acrylamide)
Loading amount
30 µg
Specification
U2OS Cells Crude Fractionation (Cytosol v Membrane
Blocking step
Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: AlexaFluor 790
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 790
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 790
Detection
Detection method
Licor / Odyssey
Negative control
Cytosolic Fraction
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 31 kDa Non-specific: 38 kDa
Positive control
Input and Membrane Fractionation
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Michael Munson
Verified customer
投稿 Sep 08 2017