Application
Western blot
Sample
Mouse Tissue lysate - other (Hippocampus)
Gel Running Conditions
Reduced Denaturing (13%)
Loading amount
6 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBS-Tween 0.1%
Dilution
1/4000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Thermo. Supersignal West Pico
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 31 kDa
Additional data
Additional Notes
Lanes 1 and 2: Hippocampus whole tissue homogenate, 2 different mouse samples.
Lanes 3 and 4: Hippocampus mitochondrial fraction, 2 different mouse samples.
Lanes 5 and 6: Hippocampus cytosolic fraction, 2 different mouse samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Sergi Bayod
Verified customer
投稿 Aug 31 2016