Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney
Blocking step
Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Sea Block
Dilution
1/100
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
Pseudo colored Green immunofluorescent staining of frozen rat kidney with purified ab14734 Anti-VDAC1 / Porin at a working dilution of 1 in 100. The nuclei are Blue after staining with Hoechst solution and a light Red counter stain was created with a methyl green staining protocol. The antigen retrieval was performed with the NxGen decloaking chamber at pH6.0 . Alexa Fluor 647 secondary was used for its better resolution and total lack of autofluorescence.
Abcam response
We would like to thank the reviewer for submitting this AbReview for ab14734. We are pleased that this antibody worked well for you in IHC-FoFr, an application that we have not tested yet. We appreciate the time and effort that the reviewer has put into testing this antibody in this application. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 20 2020