HRP Anti-VCAM1 抗体 [EPR5047] (ab195540)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR5047] to VCAM1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Conjugation: HRP
Related conjugates and formulations
製品の概要
-
製品名
HRP Anti-VCAM1 antibody [EPR5047]
VCAM1 一次抗体 製品一覧 -
製品の詳細
HRP Rabbit monoclonal [EPR5047] to VCAM1 -
由来種
Rabbit -
標識
HRP -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Mouse Brain and Rat Brain tissue lysates. IHC-P: normal human tonsil tissue sections
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.1% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5047 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
- Anti-VCAM1 antibody [EPR5047] (ab134047)
- Alexa Fluor® 647 Anti-VCAM1 antibody [EPR5047] (ab194319)
- Anti-VCAM1 antibody [EPR5047] - Low endotoxin, Azide free (ab215380)
- PE Anti-VCAM1 antibody [EPR5047] (ab223981)
- FITC Anti-VCAM1 antibody [EPR5047] (ab223982)
- APC Anti-VCAM1 antibody [EPR5047] (ab223983)
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab195540の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody. |
|
WB |
1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 81 kDa).
|
特記事項 |
---|
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. ab199507 - Rabbit monoclonal IgG (HRP), is suitable for use an as isotype control with this antibody. |
WB
1/5000. Detects a band of approximately 95 kDa (predicted molecular weight: 81 kDa). |
ターゲット情報
-
機能
Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with the beta-1 integrin VLA4 on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/VLA4 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation. -
組織特異性
Expressed on inflammed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflammed tissue. -
配列類似性
Contains 7 Ig-like C2-type (immunoglobulin-like) domains. -
ドメイン
Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion. -
翻訳後修飾
Sialoglycoprotein. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 7412 Human
- Entrez Gene: 22329 Mouse
- Entrez Gene: 25361 Rat
- GenBank: NP_001069.1 Human
- GenBank: NP_001186763.1 Human
- Omim: 192225 Human
- SwissProt: P19320 Human
- SwissProt: P29533 Mouse
see all -
別名
- CD106 antibody
- CD106 Antigen antibody
- INCAM 100 antibody
see all
画像
-
IHC image of VCAM1 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with Tris/EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab195540, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
-
All lanes : HRP Anti-VCAM1 antibody [EPR5047] (ab195540) at 1/5000 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 81 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab195540 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab195540 は論文での使用が確認できていません。