Anti-Vaspin 抗体 [EPR23080-232] - BSA and Azide free (ab270751)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23080-232] to Vaspin - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Vaspin antibody [EPR23080-232] - BSA and Azide free
Vaspin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23080-232] to Vaspin - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: IHC-Fr or IHC-P -
種交差性
交差種: Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Rat stomach, Rat kidney and Rat skin, His-tagged recombinant Rat Serpin A12 Protein lysates. IP: Rat stomach tissue lysate
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特記事項
ab270751 is the carrier-free version of ab267471.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23080-232 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab270751の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa.
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IP |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 47 kDa. |
IP
Use at an assay dependent concentration. |
ターゲット情報
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機能
May modulates insulin action conceivably only in the presence of its yet undefined target proteases in white adipose tissues. -
組織特異性
Expressed in visceral adipose tissues. -
配列類似性
Belongs to the serpin family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 145264 Human
- Entrez Gene: 191570 Rat
- SwissProt: Q8IW75 Human
- SwissProt: Q8R4Z1 Rat
- Unigene: 99476 Human
- Unigene: 79035 Rat
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別名
- ENSG00000165953 antibody
- OL 64 antibody
- OL-64 antibody
see all
画像
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Vaspin was immunoprecipitated from 0.35 mg Rat stomach tissue lysate with ab267471 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267471 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat stomach tissue lysate 10ug
Lane 2: ab267471 IP in Rat stomach tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267471 in Rat stomach tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267471).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab270751 は論文での使用が確認できていません。