Anti-VAPA 抗体 [EPR13589(B)] - N-terminal (ab181067)
![Western blot - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)](https://www.abcam.co.jp/ps/products/181/ab181067/Images/ab181067-213295-anti-vapa-antibody-epr13589b-n-terminal-western-blot.jpg "Western blot - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13589(B)] to VAPA - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-VAPA antibody [EPR13589(B)] - N-terminal
VAPA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13589(B)] to VAPA - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Orangutan
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- HeLa, K562, HEK293, HepG2 cell lysates. Human fetal colon and Human ovarian carcinoma tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR13589(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181067の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/10000 - 1/50000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
1/10000 - 1/50000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May play a role in vesicle trafficking. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.
Contains 1 MSP domain. -
細胞内局在
Endoplasmic reticulum membrane. Present in the plasma membrane and in intracellular vesicles, together with SNARE proteins. May also associate with the cytoskeleton. Colocalizes with OCLN at the tight junction in polarized epithelial cells. - Information by UniProt
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参照データベース
- Entrez Gene: 516024 Cow
- Entrez Gene: 9218 Human
- Entrez Gene: 30960 Mouse
- Entrez Gene: 100173641 Orangutan
- Entrez Gene: 58857 Rat
- Omim: 605703 Human
- SwissProt: Q0VCY1 Cow
- SwissProt: Q9P0L0 Human
see all -
別名
- 33 kDa Vamp-associated protein antibody
- hVAP 33 antibody
- MGC3745 antibody
see all
画像
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Western blot - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)All lanes : Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HEK293 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)](https://www.abcam.co.jp/ps/products/181/ab181067/Images/ab181067-213297-anti-vapa-antibody-epr13589b-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)Immunohistochemical analysis of Human fetal colon tissue, staining VAPA with ab181067 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)](https://www.abcam.co.jp/ps/products/181/ab181067/Images/ab181067-213298-anti-vapa-antibody-epr13589b-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)Immunohistochemical analysis of Human ovarian carcinoma, staining VAPA with ab181067 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)](https://www.abcam.co.jp/ps/products/181/ab181067/Images/ab181067-4-benefits-of-recombinant-antibodies.png)
Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)
プロトコール
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (3)
ab181067 は 3 報の論文で使用されています。
- Lu M et al. The structure and global distribution of the endoplasmic reticulum network are actively regulated by lysosomes. Sci Adv 6:N/A (2020). PubMed: 33328230
- Papadopoulou AA et al. Signal peptide peptidase-like 2c impairs vesicular transport and cleaves SNARE proteins. EMBO Rep 20:e46451 (2019). PubMed: 32048434
- Papadopoulou AA et al. Signal Peptide Peptidase-Like 2c (SPPL2c) impairs vesicular transport and cleavage of SNARE proteins. EMBO Rep 20:N/A (2019). PubMed: 30733281
