Anti-VAMP8/EDB 抗体 (ab6186)

Submit a review Q&A (4)References (7)

Key features and details

  • Rabbit polyclonal to VAMP8/EDB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-VAMP8/EDB antibody
    VAMP8/EDB 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to VAMP8/EDB

  • 由来種

    Rabbit

  • 特異性

    Reacts specifically with the 10 kDa protein of human CaCo-2, rat PC12 and dog MDCK cell extracts.

  • 種交差性

    交差種: Human
    交差が予測される動物種: Dog

  • 免疫原

    Synthetic peptide corresponding to Human VAMP8/EDB.

  • 特記事項

    Previously labelled as VAMP8. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

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    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

  • 追加情報
    IF: 1/50.
    IM: Use at an assay dependant dilution.
    IP: Use at an assay dependant dilution.
    WB: 1/200 - 1/1000.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    • 機能

      Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.

    • 組織特異性

      Platelets.

    • 配列類似性

      Belongs to the synaptobrevin family.
      Contains 1 v-SNARE coiled-coil homology domain.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • EDB antibody
        • Endobrevin antibody
        • VAMP 8 antibody
        • VAMP-8 antibody
        • VAMP8 antibody
        • VAMP8_HUMAN antibody
        • Vesicle associated membrane protein 8 antibody
        • Vesicle-associated membrane protein 8 antibody
        see all

      参考文献 (7)

      ab6186 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab6186 は 7 報の論文で使用されています。

      • Yang Y  et al. Polyphenols differentially inhibit degranulation of distinct subsets of vesicles in mast cells by specific interaction with granule-type-dependent SNARE complexes. Biochem J 450:537-46 (2013). PubMed: 23252429
      • Bethani I  et al. Endosomal fusion upon SNARE knockdown is maintained by residual SNARE activity and enhanced docking. Traffic 10:1543-59 (2009). WB . PubMed: 19624487
      • Mashima H  et al. Involvement of vesicle-associated membrane protein 7 in human gastric epithelial cell vacuolation induced by Helicobacter pylori-produced VacA. Infect Immun 76:2296-303 (2008). PubMed: 18362137
      • Lapierre LA  et al. Characterization of immunoisolated human gastric parietal cells tubulovesicles: identification of regulators of apical recycling. Am J Physiol Gastrointest Liver Physiol 292:G1249-62 (2007). WB, IHC-P, IF ; Human . PubMed: 17255364
      • Boström P  et al. SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated in insulin sensitivity. Nat Cell Biol 9:1286-93 (2007). PubMed: 17922004
      • Pooley RD  et al. CytLEK1 is a regulator of plasma membrane recycling through its interaction with SNAP-25. Mol Biol Cell 17:3176-86 (2006). PubMed: 16672379
      • Polgár J  et al. Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion. Blood 100:1081-3 (2002). WB ; Human . PubMed: 12130530

      レビューと Q&A

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      1-4 of 4 Abreviews or Q&A

      Question

      regarding the vamp8 polyclonal antibody. could you provide more information regarding the human n terminal immunogen of vamp8 used? was the entire cytoplasmic domain of the endobrevin molecule used? ie what residues of human vamp8 specifically . thanks

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      Answer

      Regarding the vamp8 polyclonal antibody, we could not give you the response of your questions because these informations are confidential.

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      Question

      I am interested in your VAMP3 and VANP8 antibody (Anti human) you don`t have reference for VAMP8. I wonder whether you can give me more information about this antibody

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      Answer

      I agree that the information we have on the datasheet for ab6186 is minimal, but this is all I have to hand. If you let me know your specific questions, I can ask the originator of the antibody if they can help. The same goes for ab6185 -- Rabbit antiserum to human VAMP4

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      Question

      What immunogen was used to produce this Ab? ie. what peptide sequence or site in the hVAMP8 sequence?

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      Answer

      The immunogen for this antibody was a synthetic peptide derived from the N-terminal domain of human endobrevin/VAMP8.

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      Question

      What other species does this antibody cross-react with?

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      Answer

      No cross-reactivity was found on rat PC12 and canine MDCK cell extracts. Cross reactivity in other species has not yet been tested. Specific reference: Lafont et al., PNAS 1999

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