Anti-VAMP8/EDB 抗体 (ab6186)
Key features and details
- Rabbit polyclonal to VAMP8/EDB
- Suitable for: IP, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-VAMP8/EDB antibody
VAMP8/EDB 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to VAMP8/EDB -
由来種
Rabbit -
特異性
Reacts specifically with the 10 kDa protein of human CaCo-2, rat PC12 and dog MDCK cell extracts. -
アプリケーション
適用あり: IP, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Dog
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免疫原
Synthetic peptide corresponding to Human VAMP8/EDB.
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特記事項
Previously labelled as VAMP8.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: Whole serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab6186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | ||
| ICC/IF |
追加情報
IF: 1/50.
IM: Use at an assay dependant dilution.
IP: Use at an assay dependant dilution.
WB: 1/200 - 1/1000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IM: Use at an assay dependant dilution.
IP: Use at an assay dependant dilution.
WB: 1/200 - 1/1000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes. -
組織特異性
Platelets. -
配列類似性
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8673 Human
- Omim: 603177 Human
- SwissProt: Q9BV40 Human
- Unigene: 714302 Human
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別名
- EDB antibody
- Endobrevin antibody
- VAMP 8 antibody
see all
プロトコール
データシートおよび資料
参考文献 (7)
ab6186 は 7 報の論文で使用されています。
- Yang Y et al. Polyphenols differentially inhibit degranulation of distinct subsets of vesicles in mast cells by specific interaction with granule-type-dependent SNARE complexes. Biochem J 450:537-46 (2013). PubMed: 23252429
- Bethani I et al. Endosomal fusion upon SNARE knockdown is maintained by residual SNARE activity and enhanced docking. Traffic 10:1543-59 (2009). WB . PubMed: 19624487
- Mashima H et al. Involvement of vesicle-associated membrane protein 7 in human gastric epithelial cell vacuolation induced by Helicobacter pylori-produced VacA. Infect Immun 76:2296-303 (2008). PubMed: 18362137
- Lapierre LA et al. Characterization of immunoisolated human gastric parietal cells tubulovesicles: identification of regulators of apical recycling. Am J Physiol Gastrointest Liver Physiol 292:G1249-62 (2007). WB, IHC-P, IF ; Human . PubMed: 17255364
- Boström P et al. SNARE proteins mediate fusion between cytosolic lipid droplets and are implicated in insulin sensitivity. Nat Cell Biol 9:1286-93 (2007). PubMed: 17922004
- Pooley RD et al. CytLEK1 is a regulator of plasma membrane recycling through its interaction with SNAP-25. Mol Biol Cell 17:3176-86 (2006). PubMed: 16672379
- Polgár J et al. Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion. Blood 100:1081-3 (2002). WB ; Human . PubMed: 12130530
