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    vamp2-antibody-ab3347.pdf

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Signal Transduction Protein Trafficking Vesicle Transport SNAPs & SNAREs
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Anti-VAMP2 抗体 (ab3347)

  • Datasheet
  • SDS
Reviews (3)Q&A (2)References (37)

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-VAMP2 antibody (ab3347)

    Key features and details

    • Rabbit polyclonal to VAMP2
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Cow, Human
    • Isotype: IgG

    こちらの製品もご検討ください

    二次抗体
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    タンパク質
    Product image
    Recombinant Mouse VAMP2 protein (His tag) (ab222979)
    一次抗体
    Product image
    Anti-Synaptophysin antibody [YE269] (ab32127)

    関連製品

    製品の概要

    • 製品名

      Anti-VAMP2 antibody
      VAMP2 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to VAMP2
    • 由来種

      Rabbit
    • アプリケーション

      適用あり: WBmore details
    • 種交差性

      交差種: Mouse, Rat, Cow, Human
      交差が予測される動物種: Rhesus monkey
    • 免疫原

      Synthetic peptide corresponding to Rat VAMP2 aa 1-18.
      Sequence:

      MSATAATVPPAAPAGEGG


      (Peptide available as ab4958)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      Constituents: PBS, 0.1% BSA
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Signal Transduction
      • Protein Trafficking
      • Vesicle Transport
      • SNAPs & SNAREs
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Integration of energy metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Integration of energy
      • Metabolism
      • Types of disease
      • Cancer

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Mouse VAMP2 protein (His tag) (ab222979)

    アプリケーション

    Our Abpromise guarantee covers the use of ab3347 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

    ターゲット情報

    • 機能

      Involved in the targeting and/or fusion of transport vesicles to their target membrane.
    • 組織特異性

      Nervous system and skeletal muscle.
    • 配列類似性

      Belongs to the synaptobrevin family.
      Contains 1 v-SNARE coiled-coil homology domain.
    • 細胞内局在

      Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
    • Target information above from: UniProt accession P63027 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 282116 Cow
      • Entrez Gene: 6844 Human
      • Entrez Gene: 22318 Mouse
      • Entrez Gene: 24803 Rat
      • Omim: 185881 Human
      • SwissProt: P63027 Human
      • SwissProt: P63044 Mouse
      • SwissProt: P63045 Rat
      • Unigene: 25348 Human
      • Unigene: 28643 Mouse
      • Unigene: 12939 Rat
      see all
    • 別名

      • FLJ11460 antibody
      • RATVAMPB antibody
      • RATVAMPIR antibody
      • SYB antibody
      • SYB2 antibody
      • Synaptobrevin 2 antibody
      • Synaptobrevin-2 antibody
      • VAMP 2 antibody
      • VAMP-2 antibody
      • Vamp2 antibody
      • VAMP2_HUMAN antibody
      • Vesicle associated membrane protein 2 antibody
      • Vesicle-associated membrane protein 2 (synaptobrevin 2) antibody
      • Vesicle-associated membrane protein 2 antibody
      see all

    画像

    • Western blot - Anti-VAMP2 antibody (ab3347)
      Western blot - Anti-VAMP2 antibody (ab3347)

      Western blot detection of VAMP2 on rat brain extract using ab3347.

    プロトコール

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (37)

    ab3347 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3347 は 37 報の論文で使用されています。

    • Kokotos AC  et al. Synaptophysin sustains presynaptic performance by preserving vesicular synaptobrevin-II levels. J Neurochem 151:28-37 (2019). PubMed: 31216055
    • Brandenburger T  et al. MiR-34a is differentially expressed in dorsal root ganglia in a rat model of chronic neuropathic pain. Neurosci Lett 708:134365 (2019). PubMed: 31278961
    • Joussain C  et al. Botulinum Neurotoxin Light Chains Expressed by Defective Herpes Simplex Virus Type-1 Vectors Cleave SNARE Proteins and Inhibit CGRP Release in Rat Sensory Neurons. Toxins (Basel) 11:N/A (2019). PubMed: 30791373
    • Hoffman BU  et al. Merkel Cells Activate Sensory Neural Pathways through Adrenergic Synapses. Neuron 100:1401-1413.e6 (2018). PubMed: 30415995
    • Actis Dato V  et al. Insulin-induced exocytosis regulates the cell surface level of low-density lipoprotein-related protein-1 in Müller Glial cells. Biochem J 475:1669-1685 (2018). PubMed: 29669912
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-VAMP2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    2 µg
    Gel Running Conditions
    Non-reduced Denaturing
    Sample
    Rat Tissue lysate - whole (hippocampus tissue lysate)
    Specification
    hippocampus tissue lysate
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 07 2014

    Question

    In hopes of answering your questions:

    1) the problem is we are not seeing any bands even in a control sample prepared from kidney cortex.
    2) We are using protein samples from IMCD3 cell lystates. These were prepared using a RIPA lysis buffer.
    3) The primary antibody is being incubated overnight at 4 degrees Celsius.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 05 2012

    Answer

    Thank you for your reply and the additional information.

    Please let me know your order or PO number. If the antibody was purchased within the last 6 months, our Abpromise guarantee applies and I'd be happy to offer you either a free of charge replacement, a credit or a refund.

    Please let me know which option would be best for you.

    I look forward to hear back and resolve this case for you.

    Read More

    Abcam Scientific Support

    Answered on Nov 05 2012

    Question

    We have attempted to use your Anti-VAMP2 antibody (ab3347) for Western Blotting. We have unfortunately had no success working with a 1:500 dilution. Is there a suggested protocol that you may be able to offer?

    Here are some highlights of our procedure:

    - 100ug protein loaded
    - 15 minute denaturation at 75 degrees Celsius
    - 1 Hour block with 10% Non Fat Dry Skim Milk (NFDSM)
    - 1:500 Anti-VAMP2 in 10% NFDSM
    - 90 min 1:2000 Secondary
    - 30 min ECL development

    Read More

    Abcam community

    Verified customer

    Asked on Nov 02 2012

    Answer

    Thank you for contacting us.
    I am sorry to hear that the antibody is not working as expected.

    I have copied the WB protocol below.
    Also,
    I have a few more questions in order to understand the issue better:
    1) What is the exact problem (e.g. no bands, high background)?
    2) What type of samples have you used and how were they prepared?
    3) How long did you incubate with the primary antibody?



    Western Blot Protocol

    Polyacrylamide gel electrophoresis and blottingAdd an appropriate amount of electrophoresis sample buffer (1X = 125mM Tris-HCl pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, and 1% beta-mercaptoethanol) to all samples.
    Heat to 95°C for 3-5 minutes.
    Load 5-100 ug total protein in a volume that is appropriate for the size of the wells.
    Electrophorese proteins for the appropriate time according to the manufacturers instructions.
    Transfer proteins from the gel to a suitable membrane (e.g. nitrocellulose, PVDF, etc.) following the manufacturers protocol for transfer.
    High molecular weight proteins (>120 kDa) can be wet transferred more efficiently if transfer time is increased and 0.05% SDS is included in the transfer buffer.


    BlockingRemove the filter from the transfer apparatus and rinse in PBST/TBST to remove loose acrylamide.
    Transferred proteins can be visualized by staining the membrane for 15-30 minutes with Ponceau S.
    Remove stain from filter by washing with PBST/TBST.
    Place filter into blocking solution.
    Block for 30 minutes at 37°C, 1 hour at room temperature, or overnight at 4°C.


    Incubation with primary antibodyDecant the blocking buffer and add the antibody, diluted in blocking buffer as suggested in the product description sheet.
    Incubate with agitation for 30 minutes at 37°C, 1 hour at room temperature, or overnight at 4°C.


    Incubation with secondary antibodyWash for 30 minutes with agitation in wash buffer (TBS or PBS with 0.1% Tween 20), changing the wash buffer every 5 minutes.
    Decant the wash solution and add AffiniClear HRP-conjugated secondary antibody, diluted in 5% non-fat dry milk in wash buffer.
    Incubate for 30 minutes at 37°C, 1 hour at room temperature, or overnight at 4°C.
    Decant the antibody conjugate and wash for 30 minutes with agitation in wash buffer (TBS or PBS with 0.1% Tween 20), changing the wash buffer every 5 minutes.


    Substrate incubation (ECL)Decant washing buffer and place the blot in a plastic bag or clean tray containing the development working solution (0.125 ml/cm2) for 1-5 minutes.
    Agitate bag or tray to cover the surface of the membrane.
    Remove the blot from the bag or tray and place it between two pieces of write-on transparency film.
    Smooth over the covered blot to remove air bubbles and excess substrate.
    Expose to X-ray film or any sensitive screen. (Check manufacturer's instructions for specific ECL reagents and procedures.)


    I look forward to hear back from you and assist you further.

    Read More

    Abcam Scientific Support

    Answered on Nov 02 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-VAMP2 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Read More

    Dr. Karine Thibault

    Verified customer

    投稿 Apr 14 2009

    Immunocytochemistry/ Immunofluorescence abreview for Anti-VAMP2 antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (hippocampal neuron)
    Specification
    hippocampal neuron
    Fixative
    Paraformaldehyde
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 2%
    Read More

    Dr. Chanchoo Yap

    Verified customer

    投稿 Nov 27 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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