Western blot abreview for Anti-V5 tag antibody (HRP)

Excellent
Abreviews
Application
Western blot
Loading amount
0.1 µg
Gel Running Conditions
Reduced Denaturing (4~20%)
Sample
Escherichia coli Recombinant protein (Recombinant protein with C-terminus V5 tag)
Specification
Recombinant protein with C-terminus V5 tag
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Diluent: TBST+5%Milk
Dilution
1/2000

Secondary antibody

Secondary antibody
None used

Detection

Detection method
ECL+
Bands
Specific: 52 kDa
Exposure
15 second(s)
Positive control
None

Abcam user community

Verified customer

投稿 May 30 2014

登録