Application
Western blot
Sample
Mouse Cell lysate - whole cell (ES cells)
Loading amount
30 µg
Specification
ES cells
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/5000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit Ig, Horse radish peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
Millipore ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 72 kDa
Additional data
Additional Notes
Lanes 1-3 cells transfected with v5-tagged protein
Lanes 4-5 untransfected cells
Lanes 4-5 untransfected cells
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 13 2009