Anti-USP51 抗体 (ab121147)
Key features and details
- Rabbit polyclonal to USP51
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-USP51 antibody -
製品の詳細
Rabbit polyclonal to USP51 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Horse, Pig, Gorilla -
免疫原
Recombinant fragment: LRLIYQRFVW SGTPETRKRK AKSCICHVCS THMNRLHSCL SCVFFGCFTE KHIHKHAETK QHHLAVDLYH GVIYCFMCKD YVYDKDIEQI AKETKEKILR LLTSTSTDVS HQQFMTSGFE DKQSTCETKE QEP, corresponding to internal sequence amino acids 211-343 of Human USP51 (UniProt ID: Q70EK9).
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ポジティブ・コントロール
- IHC-P: Human pancreas tissue WB: Human RT-4, U-251 MG, A-431, liver, and tonsil lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab121147の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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組織特異性
Expressed in prostate, brain, lung, aorta and kidney. -
配列類似性
Belongs to the peptidase C19 family.
Contains 1 UBP-type zinc finger. - Information by UniProt
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参照データベース
- Entrez Gene: 158880 Human
- SwissProt: Q70EK9 Human
- Unigene: 40061 Human
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別名
- Deubiquitinating enzyme 51 antibody
- Ubiquitin carboxyl-terminal hydrolase 51 antibody
- Ubiquitin specific peptidase 51 antibody
see all
画像
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Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab121147 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemical staining of paraffin embedded Human pancreas tissue, using ab121147 at a 1/100 dilution. The image shows strong cytoplasmic positivity in exocrine glandular cells.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab121147 は 2 報の論文で使用されています。
- Che X et al. FAT4-USP51 complex regulates the proliferation and invasion of endometrial cancer via Hippo pathway. Am J Transl Res 11:2784-2800 (2019). PubMed: 31217854
- Zhou Z et al. USP51 promotes deubiquitination and stabilization of ZEB1. Am J Cancer Res 7:2020-2031 (2017). PubMed: 29119051